CAJ | 학술논문

为探明烟草内生细菌种群特征及其与烟草的关系，采用研磨组织稀释分离法分离不同品种、不同生长期、不同器官的烟草内生细菌，以各菌群出现与否为指标，用类平均法进行系统聚类分析，对内生细菌的种类组成，分布特征进行了研究。结果表明，烟草内生细菌种类组成存在品种差异、生长期差异和组织部位差异；优势菌群随分离部位和生长时期的不同而变化。Bacillus sp.和Rhizobiumsp.是4个品种烟草叶片共有的优势类群，从苗期到团棵期，内生细菌的种类在增加，B. pumilus广泛存在于成熟期烟草的根、茎、叶中，且为主要菌群。部分内生细菌具有组织偏好性，Micrococcus sp.和Staphylococcus sp.对烟草的茎部具有偏好性，B. cereus对烟草叶片具有偏好性。研究结果为烟草有益内生细菌的筛选奠定了基础，并为内生细菌在其它植物中的开发利用提供理论依据。
위탐명연초내생세균충군특정급기여연초적관계，채용연마조직희석분리법분리불동품충、불동생장기、불동기관적연초내생세균，이각균군출현여부위지표，용류평균법진행계통취류분석，대내생세균적충류조성，분포특정진행료연구。결과표명，연초내생세균충류조성존재품충차이、생장기차이화조직부위차이；우세균군수분리부위화생장시기적불동이변화。Bacillus sp.화Rhizobiumsp.시4개품충연초협편공유적우세류군，종묘기도단과기，내생세균적충류재증가，B. pumilus엄범존재우성숙기연초적근、경、협중，차위주요균군。부분내생세균구유조직편호성，Micrococcus sp.화Staphylococcus sp.대연초적경부구유편호성，B. cereus대연초협편구유편호성。연구결과위연초유익내생세균적사선전정료기출，병위내생세균재기타식물중적개발이용제공이론의거。
Endophytic bacteria were isolated from tobacco by the method of grinding separation. The composition of endophytic bacteria population in different cultivars, different tissues and different growth stages were studied. Results showed that the composition and quantity of dominant bacterial community varied with changes of cultivars, growth stages and tissues.Bacillus sp. andRhizobium sp. were considered as dominant genera of endophytic bacteria in leaf from different tobacco cultivars. The endophytic bacteria flora in leaf increased from seedling stage to maturing stage.B. pumilus were considered as dominant genera of endophytic bacteria in root, stem and leaf during maturing stage. Cluster analysis in which euclidean distance was taken as similarity measure using group average method showed that endophytic bacteria in tobacco had tissue specificity.Micrococcus sp. andStaphylococcus sp. had tissue specificity to stem whileBacillus cereus had tissue specificity to leaf. The study could help to lay foundation for screening beneficial endophytic bacteria and to provide references for using in other crop plants.