中国烟草学报
中國煙草學報
중국연초학보
ACTA TABACARIA SINICA
2014年
3期
79-83
,共5页
焦永吉%王闷灵%赵云波%赵世民%张涵%蒋士君
焦永吉%王悶靈%趙雲波%趙世民%張涵%蔣士君
초영길%왕민령%조운파%조세민%장함%장사군
根结线虫%PCR分子检测%会阴花纹鉴定
根結線蟲%PCR分子檢測%會陰花紋鑒定
근결선충%PCR분자검측%회음화문감정
root-knot nematode (Meloidogyne spp.)%PCR molecular detection%perineal pattern identification
为明确河南省烟田根结线虫种群分布及比例，采集了12份烟草根结线虫样本。通过对线虫样本的PCR分子检测和雌成虫会阴花纹的形态鉴定，结果表明，检出烟草根结线虫4种，其中南方根结线虫（Meloidogyne incognita）检出率为55.83%，花生根结线虫（Meloidogyne arenaria）23.33%，北方根结线虫（Meloidogyne hapla）17.50%；爪哇根结线虫（Meloidogyne javanica）3.33%。种群分析表明河南地区仍以南方根结线虫为主，同时其他种类根结线虫的发生危害比例在上升。所有样本均为混合侵染，南方根结线虫、花生根结线虫和北方根结线虫在河南广泛分布，具有普遍性；爪哇根结线虫在河南分布没有规律性。
為明確河南省煙田根結線蟲種群分佈及比例，採集瞭12份煙草根結線蟲樣本。通過對線蟲樣本的PCR分子檢測和雌成蟲會陰花紋的形態鑒定，結果錶明，檢齣煙草根結線蟲4種，其中南方根結線蟲（Meloidogyne incognita）檢齣率為55.83%，花生根結線蟲（Meloidogyne arenaria）23.33%，北方根結線蟲（Meloidogyne hapla）17.50%；爪哇根結線蟲（Meloidogyne javanica）3.33%。種群分析錶明河南地區仍以南方根結線蟲為主，同時其他種類根結線蟲的髮生危害比例在上升。所有樣本均為混閤侵染，南方根結線蟲、花生根結線蟲和北方根結線蟲在河南廣汎分佈，具有普遍性；爪哇根結線蟲在河南分佈沒有規律性。
위명학하남성연전근결선충충군분포급비례，채집료12빈연초근결선충양본。통과대선충양본적PCR분자검측화자성충회음화문적형태감정，결과표명，검출연초근결선충4충，기중남방근결선충（Meloidogyne incognita）검출솔위55.83%，화생근결선충（Meloidogyne arenaria）23.33%，북방근결선충（Meloidogyne hapla）17.50%；조왜근결선충（Meloidogyne javanica）3.33%。충군분석표명하남지구잉이남방근결선충위주，동시기타충류근결선충적발생위해비례재상승。소유양본균위혼합침염，남방근결선충、화생근결선충화북방근결선충재하남엄범분포，구유보편성；조왜근결선충재하남분포몰유규률성。
Root-knot nematode is one of the most important diseases in tobacco in Henan province.12 samples were collected from 8 counties from main tobacco planting areas. Perineal pattern-based morphological and PCR-basedmolecular identificationrevealed that 4 species of Meloidogynewere detected. of whichM. incognita accounted for 55.83%,M. arenaria23.33%,M. hapla17.50% andM. javanica 3.33%.Meloidogyne incognita was dominant although other species in the pathogen population of tobacco root-knot nematode were increasing.All samples showed mixed infection.M. incognita,M. arenaria andM. hapla were very common and widely distributed in Henan whileM. javanicadistributed unevenly.
