CAJ | 학술논문

为明确河南省烟田根结线虫种群分布及比例，采集了12份烟草根结线虫样本。通过对线虫样本的PCR分子检测和雌成虫会阴花纹的形态鉴定，结果表明，检出烟草根结线虫4种，其中南方根结线虫（Meloidogyne incognita）检出率为55.83%，花生根结线虫（Meloidogyne arenaria）23.33%，北方根结线虫（Meloidogyne hapla）17.50%；爪哇根结线虫（Meloidogyne javanica）3.33%。种群分析表明河南地区仍以南方根结线虫为主，同时其他种类根结线虫的发生危害比例在上升。所有样本均为混合侵染，南方根结线虫、花生根结线虫和北方根结线虫在河南广泛分布，具有普遍性；爪哇根结线虫在河南分布没有规律性。
위명학하남성연전근결선충충군분포급비례，채집료12빈연초근결선충양본。통과대선충양본적PCR분자검측화자성충회음화문적형태감정，결과표명，검출연초근결선충4충，기중남방근결선충（Meloidogyne incognita）검출솔위55.83%，화생근결선충（Meloidogyne arenaria）23.33%，북방근결선충（Meloidogyne hapla）17.50%；조왜근결선충（Meloidogyne javanica）3.33%。충군분석표명하남지구잉이남방근결선충위주，동시기타충류근결선충적발생위해비례재상승。소유양본균위혼합침염，남방근결선충、화생근결선충화북방근결선충재하남엄범분포，구유보편성；조왜근결선충재하남분포몰유규률성。
Root-knot nematode is one of the most important diseases in tobacco in Henan province.12 samples were collected from 8 counties from main tobacco planting areas. Perineal pattern-based morphological and PCR-basedmolecular identificationrevealed that 4 species of Meloidogynewere detected. of whichM. incognita accounted for 55.83%,M. arenaria23.33%,M. hapla17.50% andM. javanica 3.33%.Meloidogyne incognita was dominant although other species in the pathogen population of tobacco root-knot nematode were increasing.All samples showed mixed infection.M. incognita,M. arenaria andM. hapla were very common and widely distributed in Henan whileM. javanicadistributed unevenly.