CAJ | 학술논문

为寻找与烟叶TSNA含量显著关联的标记位点，利用分布于烟草24个染色体具多态性的SSR标记和MFLP标记，分析24份烟草种质材料的遗传多样性，在此基础上对烟叶中烟草特有亚硝胺（TSNA）含量的表型变异与标记多态性进行关联分析。结果显示，33对MFLP引物和28对SSR引物在24份烟草材料中共发现188个多态位点；群体结构分析将24份烟草材料分为3个亚群，且亚群划分与烟草类型（烤烟、晾晒烟和白肋烟）基本一致；关联分析发现1个SSR位点和6个MFLP位点至少与1种TSNA含量的相关性在0.01水平上显著，其中标记MFLP26与烤后烟叶中NNN含量的相关性在0.001水平上显著，表型变异解释率最高（R2=0.5831）。这些显著关联的分子标记可为筛选低TSNA含量的烟草材料提供参考。
위심조여연협TSNA함량현저관련적표기위점，이용분포우연초24개염색체구다태성적SSR표기화MFLP표기，분석24빈연초충질재료적유전다양성，재차기출상대연협중연초특유아초알（TSNA）함량적표형변이여표기다태성진행관련분석。결과현시，33대MFLP인물화28대SSR인물재24빈연초재료중공발현188개다태위점；군체결구분석장24빈연초재료분위3개아군，차아군화분여연초류형（고연、량쇄연화백륵연）기본일치；관련분석발현1개SSR위점화6개MFLP위점지소여1충TSNA함량적상관성재0.01수평상현저，기중표기MFLP26여고후연협중NNN함량적상관성재0.001수평상현저，표형변이해석솔최고（R2=0.5831）。저사현저관련적분자표기가위사선저TSNA함량적연초재료제공삼고。
Genetic diversity analysis for 24 tobacco accessions was performed with SSR and MFLP markers which distributed in 24 chromosomes. Association analysis was conducted between polymorphic markers and tobacco specific nitrosamine (TSNA) contents through GLM model using software TASSEL. Result showed that 188 allelic variations were detected among 24 tobacco accessions with 33 MFLP and 28 SSR primer combinations. These accessions were clustered into 3 subgroups in genetic analysis of population structure. The subgroup is in coincidence with tobacco types, i.e. flue-cured, sun-cured and burley. Association analysis showed that 6 MFLP markers and 1 SSR marker were associated with at least one of TSNA contents at P<0.01 level. The marker of MFLP26 was significantly associated with NNN content (P<0.001), and explains 58.31% of phenotypic variation for NNN content in cured leaves. These markers could be used in tobacco breeding programmes to assist with selection of tobacco genotypes with low TSNA content.