国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2014年
10期
1378-1381
,共4页
林荣华%綦巧雯%林明祥%王宪耀%郭予涛
林榮華%綦巧雯%林明祥%王憲耀%郭予濤
림영화%기교문%림명상%왕헌요%곽여도
原发性肾病综合症%儿童%IgE%CD23
原髮性腎病綜閤癥%兒童%IgE%CD23
원발성신병종합증%인동%IgE%CD23
Primary nephrotic syndrome%children%IgE%CD23
目的 探讨外周血中IgE和CD23表达水平与儿童原发性肾病综合症的相关性.方法 采用免疫放射法和流式细胞技术检测52例肾病综合症患儿以及13例健康儿童的血清IgE水平和CD23阳性细胞率.结果 肾病综合症患儿的IgE表达水平和CD23阳性细胞率均明显高于对照组的健康儿童,IgE水平:(452.5±239.7) vs.(132.6±105.5),P＜0.01; CD23阳性率:(16.6±7.6)vs.(5.2±3.9),P＜ 0.05,差异具有统计学意义.激素耐药型患儿的IgE的表达水平在治疗前、后均明显高于激素敏感型的患儿,治疗前:(462.9±321.2)vs.(382.1±269.0),P＜0.05;治疗后:(246.7±182.1)vs.(171.7±107.9),P＜0.05.激素耐药型患儿的CD23阳性细胞率在治疗前、后均明显高于激素敏感型的患儿,治疗前:(19.3±6.4)vs.(15.6土7.7),P＜0.05;治疗后:(12.2±8.5)vs.(6.1± 4.3),P＜ 0.01.并且,患儿的IgE水平与CD23阳性率呈正相关(r=0.605,P＜0.05).结论 原发性肾病综合症的患儿血清IgE表达和CD23阳性细胞的免疫功能紊乱,且与激素敏感型患儿相比,激素耐药型患儿的免疫功能紊乱在治疗前、后均更明显.联合检测外周血IgE水平和CD23阳性细胞率在儿童原发性肾病综合症的诊断和预后判断中有重要意义.
目的 探討外週血中IgE和CD23錶達水平與兒童原髮性腎病綜閤癥的相關性.方法 採用免疫放射法和流式細胞技術檢測52例腎病綜閤癥患兒以及13例健康兒童的血清IgE水平和CD23暘性細胞率.結果 腎病綜閤癥患兒的IgE錶達水平和CD23暘性細胞率均明顯高于對照組的健康兒童,IgE水平:(452.5±239.7) vs.(132.6±105.5),P＜0.01; CD23暘性率:(16.6±7.6)vs.(5.2±3.9),P＜ 0.05,差異具有統計學意義.激素耐藥型患兒的IgE的錶達水平在治療前、後均明顯高于激素敏感型的患兒,治療前:(462.9±321.2)vs.(382.1±269.0),P＜0.05;治療後:(246.7±182.1)vs.(171.7±107.9),P＜0.05.激素耐藥型患兒的CD23暘性細胞率在治療前、後均明顯高于激素敏感型的患兒,治療前:(19.3±6.4)vs.(15.6土7.7),P＜0.05;治療後:(12.2±8.5)vs.(6.1± 4.3),P＜ 0.01.併且,患兒的IgE水平與CD23暘性率呈正相關(r=0.605,P＜0.05).結論 原髮性腎病綜閤癥的患兒血清IgE錶達和CD23暘性細胞的免疫功能紊亂,且與激素敏感型患兒相比,激素耐藥型患兒的免疫功能紊亂在治療前、後均更明顯.聯閤檢測外週血IgE水平和CD23暘性細胞率在兒童原髮性腎病綜閤癥的診斷和預後判斷中有重要意義.
목적 탐토외주혈중IgE화CD23표체수평여인동원발성신병종합증적상관성.방법 채용면역방사법화류식세포기술검측52례신병종합증환인이급13례건강인동적혈청IgE수평화CD23양성세포솔.결과 신병종합증환인적IgE표체수평화CD23양성세포솔균명현고우대조조적건강인동,IgE수평:(452.5±239.7) vs.(132.6±105.5),P＜0.01; CD23양성솔:(16.6±7.6)vs.(5.2±3.9),P＜ 0.05,차이구유통계학의의.격소내약형환인적IgE적표체수평재치료전、후균명현고우격소민감형적환인,치료전:(462.9±321.2)vs.(382.1±269.0),P＜0.05;치료후:(246.7±182.1)vs.(171.7±107.9),P＜0.05.격소내약형환인적CD23양성세포솔재치료전、후균명현고우격소민감형적환인,치료전:(19.3±6.4)vs.(15.6토7.7),P＜0.05;치료후:(12.2±8.5)vs.(6.1± 4.3),P＜ 0.01.병차,환인적IgE수평여CD23양성솔정정상관(r=0.605,P＜0.05).결론 원발성신병종합증적환인혈청IgE표체화CD23양성세포적면역공능문란,차여격소민감형환인상비,격소내약형환인적면역공능문란재치료전、후균경명현.연합검측외주혈IgE수평화CD23양성세포솔재인동원발성신병종합증적진단화예후판단중유중요의의.
Objective To explore the correlation between the expressions of IgE and CD23 and primary nephrotic syndrome in children.Methods 52 children with primary nephrotic syndrome and 13 healthy children were recruited into this study.The serum IgE concentrations were detected by immunoradiation assay.The proportion of CD23 positive cells were determined by flow cytometry analysis.Results The IgE concentration and the proportion of CD23+ cells in the children with primary nephrotic syndrome were significantly higher than those of the health counterparts (IgE:[452.5 ± 239.7] vs.[132.6 ± 105.5],P ＜ 0.01; CD23+ cells％:[16.6 ± 7.6] vs.[5.2 ± 3.9],P ＜ 0.05).The serum IgE concentration was obviously higher in the patients with steroid-resistant nephrotic syndrome (SRNS) than in the children with steroid-sensitive nephrotic syndrome (SSNS) before and after the treatment (before the treatment:[462.9 ± 321.2] vs.[382.1 ± 269.0],P ＜ 0.05; after the treatment:[246.7 ± 182.1] vs.[171.7 ± 107.9],P ＜ 0.05).The proportion of CD23+ cells was significantly enhanced in the SRNS children than in the SSNS children before and after the treatment (before the treatment:[19.3 ± 6.4] vs.[15.6 ± 7.7],P ＜ 0.05; after the treatment:[12.2 ± 8.5] vs.[6.1 ± 4.3],P ＜ 0.01).IgE concentration positively correlated with CD23+ proportion in the children with primary nephrotic syndrome (r=0.605,P ＜ 0.05).Conclusions Immune dysfunction was observed in children with primary nephrotic syndrome,as indicated by the abnormal expression of serum IgE concentration and CD23+ cell proportion.Compared to SSNS children,these disorders were more obvious in SRNS children befaore and after treatment.Therefore,a combined testing of serum IgE concentration and CD23+ cell proportion shows great significance in the diagnosis and prognosis evaluation in primary nephrotic syndrome in children.
