中国烟草科学
中國煙草科學
중국연초과학
CHINESE TOBACCO SCIENCE
2014年
3期
61-66
,共6页
杨欢%余君%王昌军%李进平%施河丽%谭军%李锡宏%王瑞%徐迪红%陈守文
楊歡%餘君%王昌軍%李進平%施河麗%譚軍%李錫宏%王瑞%徐迪紅%陳守文
양환%여군%왕창군%리진평%시하려%담군%리석굉%왕서%서적홍%진수문
解淀粉芽胞杆菌%茄科雷尔氏菌%定殖%抗菌物质%理化性质
解澱粉芽胞桿菌%茄科雷爾氏菌%定殖%抗菌物質%理化性質
해정분아포간균%가과뢰이씨균%정식%항균물질%이화성질
Bacillus amyloliquefaciens%Ralstonia solanacearum%colonization%antibiotic substance%physicochemical characteristic
通过菌株生态定殖以及抗病物质分析，初步探究解淀粉芽胞杆菌（Bacillus amyloliquefaciens）YH-22抗青枯病（茄科雷尔氏菌所致）的机制。采用利福平抗性诱导，获得抗400μg/mL的抗性菌株以利于定殖研究。盆栽试验结果表明，YH-22先在烟草根际土壤定殖，然后依次进入根部、茎部。对 YH-22菌株发酵上清液进行理化性质分析，发现抗茄科雷尔氏菌活性物质不溶于氯仿和乙酸乙酯，能溶于甲醇，具有一定的耐热性，能耐受胃蛋白酶和紫外线，具有排油和使液滴坍塌的特性。综上所述，生防菌YH-22在烟草根际土壤、根、茎均具有一定的定殖能力，其抗菌物质可能为蛋白质和脂肽的混合物。
通過菌株生態定殖以及抗病物質分析，初步探究解澱粉芽胞桿菌（Bacillus amyloliquefaciens）YH-22抗青枯病（茄科雷爾氏菌所緻）的機製。採用利福平抗性誘導，穫得抗400μg/mL的抗性菌株以利于定殖研究。盆栽試驗結果錶明，YH-22先在煙草根際土壤定殖，然後依次進入根部、莖部。對 YH-22菌株髮酵上清液進行理化性質分析，髮現抗茄科雷爾氏菌活性物質不溶于氯倣和乙痠乙酯，能溶于甲醇，具有一定的耐熱性，能耐受胃蛋白酶和紫外線，具有排油和使液滴坍塌的特性。綜上所述，生防菌YH-22在煙草根際土壤、根、莖均具有一定的定殖能力，其抗菌物質可能為蛋白質和脂肽的混閤物。
통과균주생태정식이급항병물질분석，초보탐구해정분아포간균（Bacillus amyloliquefaciens）YH-22항청고병（가과뢰이씨균소치）적궤제。채용리복평항성유도，획득항400μg/mL적항성균주이리우정식연구。분재시험결과표명，YH-22선재연초근제토양정식，연후의차진입근부、경부。대 YH-22균주발효상청액진행이화성질분석，발현항가과뢰이씨균활성물질불용우록방화을산을지，능용우갑순，구유일정적내열성，능내수위단백매화자외선，구유배유화사액적담탑적특성。종상소술，생방균YH-22재연초근제토양、근、경균구유일정적정식능력，기항균물질가능위단백질화지태적혼합물。
Through the analysis of ecological colonization and antimicrobial substances of Bacillus amyloliquefaciens YH-22, the resistance mechanism of YH-22 against bacterial wilt which was caused by Ralstonia solanacearum was preliminarily investigated. The tolerance dosage of YH-22 resistance strain against rifampicin reached 400μg/mL by resistance induction with rifampicin. The results of pot experiments showed that YH-22 resistance strain firstly colonized on the rhizosphere soil. Then, it intruded into the root and stem successively. The antimicrobial substances in broth could be dissolved by methanol, but not by chloroform and ethyl acetate. They could maintain high stability under the conditions of heat treatment, protease hydrolysis and Ultraviolet. Moreover, they had the character of oil displacement and droplet collapse. In conclusion, strain YH-22 has colonization ability in rhizosphere soil as well as tobacco tissue. The antibacterial components of strain YH-22 may consist of proteins and lipopeptides.
