CAJ | 학술논문

以赤星病原菌Alternaria longipes为筛选指示菌，采用平板共培养初筛和发酵上清滤液复筛的方法，筛选了烟草赤星病拮抗芽胞杆菌3株。其中菌株K11的菌体径向生长抑制率（PIRG）为63.64%，发酵上清滤液的PIRG值达67.44%。盆栽试验证明，该菌对烟草赤星病具有良好的拮抗能力。经鉴定菌株K11为枯草芽胞杆菌（Bacillus subtilis）。由单因素实验和正交实验优化得到了K11菌株生物量较高的摇瓶发酵培养基配方：葡萄糖20.0 g/L，豆粕30.0 g/L，K2HPO4x3H2O 1.0 g/L， MgSO4x7H2O 0.1 g/L。优化后生防菌株K11的生物量比对照提高6.5倍，生物量高达1.28×1010 CFU/mL，芽胞形成率在95%以上。
이적성병원균Alternaria longipes위사선지시균，채용평판공배양초사화발효상청려액복사적방법，사선료연초적성병길항아포간균3주。기중균주K11적균체경향생장억제솔（PIRG）위63.64%，발효상청려액적PIRG치체67.44%。분재시험증명，해균대연초적성병구유량호적길항능력。경감정균주K11위고초아포간균（Bacillus subtilis）。유단인소실험화정교실험우화득도료K11균주생물량교고적요병발효배양기배방：포도당20.0 g/L，두박30.0 g/L，K2HPO4x3H2O 1.0 g/L， MgSO4x7H2O 0.1 g/L。우화후생방균주K11적생물량비대조제고6.5배，생물량고체1.28×1010 CFU/mL，아포형성솔재95%이상。
Three strains were selected as the Bacillus agtagonists against tobacco brown spot disease from 93 strains stored in our lab, the strain K11 showed the highest percentage inhibition of radial growth (PIRG). The PIRG was 63.64%by the dual culture while 67.44% in culture filtrate tests. According to the physiological and biochemical characteristics, the strain K11 was identified as Bacillus subtilis. Based on the single factor experiments and orthogonal experiments for K11, an optimum fermentation medium for biomass production in flasks composed of glucose 20.0 g/L, soybean meal 30.0 g/L, K2HPO4x3H2O 1.0 g/L, and MgSO4x7H2O 0.1 g/L. Under the optimum fermentation conditions, the highest biomass yield was 1.28×1010 CFU/mL, which was 6.5 times of the initial KMB medium, and the sporulation rate reached as much as 95%.