中国小儿血液与肿瘤杂志
中國小兒血液與腫瘤雜誌
중국소인혈액여종류잡지
JOURNAL OF CHINA PEDIATRIC BLOOD AND CANCER
2014年
3期
129-133,142
,共6页
环磷酰胺%吡柔比星%横纹肌肉瘤%趋化因子受体4
環燐酰胺%吡柔比星%橫紋肌肉瘤%趨化因子受體4
배린선알%필유비성%횡문기육류%추화인자수체4
Pirarubicin%Cyclophosphamide%Rhabdomyosarcoma%Chemokine CXC motif receptor 4
目的:观察敏感剂量下环磷酰胺(CTX)和吡柔比星(THP)对体外培养人横纹肌肉瘤RD细胞株CXCR4基因表达的影响。方法体外培养RD细胞株;MTT试验明确CTX和THP对RD细胞的效应剂量;细胞划痕实验检测RD细胞的迁移能力;RT- PCR及Western blot法检测RD细胞CXCR4表达。结果(1)敏感剂量下CTX(30 mmol/L)和THP(1000 ng/mL)均可抑制RD细胞的迁移(P<0.05),两药联合应用对细胞迁移抑制作用增强,较对照组差异有非常显著性(P<0.01);(2)各药物处理组(CTX,THP,CTX+THP)药物作用于RD细胞24 h后CXCR4蛋白的表达较对照组减少,其差异均有显著性(P<0.05);其中CTX+THP组较CTX组CXCR4蛋白表达减少更明显(P<0.05),但与THP组比较,差异无显著性(P>0.05);(3)同样条件下,各药物处理组(CTX,THP,CTX+THP)与对照组比较,RD细胞CXCR4 mRNA 的表达均减少,差异均有显著性(P<0.05);CTX+THP组较CTX组CXCR4 mRNA表达减少明显(P<0.05),但较THP组差异无显著性(P>0.05)。结论化疗药物CTX和THP均能抑制人横纹肌肉瘤RD细胞的增殖;敏感剂量下的两种药物均能抑制RD细胞的迁移及下调RD细胞CXCR4的表达,提示两种化疗药物发挥作用的机制可能涉及到CXCR4基因。
目的:觀察敏感劑量下環燐酰胺(CTX)和吡柔比星(THP)對體外培養人橫紋肌肉瘤RD細胞株CXCR4基因錶達的影響。方法體外培養RD細胞株;MTT試驗明確CTX和THP對RD細胞的效應劑量;細胞劃痕實驗檢測RD細胞的遷移能力;RT- PCR及Western blot法檢測RD細胞CXCR4錶達。結果(1)敏感劑量下CTX(30 mmol/L)和THP(1000 ng/mL)均可抑製RD細胞的遷移(P<0.05),兩藥聯閤應用對細胞遷移抑製作用增彊,較對照組差異有非常顯著性(P<0.01);(2)各藥物處理組(CTX,THP,CTX+THP)藥物作用于RD細胞24 h後CXCR4蛋白的錶達較對照組減少,其差異均有顯著性(P<0.05);其中CTX+THP組較CTX組CXCR4蛋白錶達減少更明顯(P<0.05),但與THP組比較,差異無顯著性(P>0.05);(3)同樣條件下,各藥物處理組(CTX,THP,CTX+THP)與對照組比較,RD細胞CXCR4 mRNA 的錶達均減少,差異均有顯著性(P<0.05);CTX+THP組較CTX組CXCR4 mRNA錶達減少明顯(P<0.05),但較THP組差異無顯著性(P>0.05)。結論化療藥物CTX和THP均能抑製人橫紋肌肉瘤RD細胞的增殖;敏感劑量下的兩種藥物均能抑製RD細胞的遷移及下調RD細胞CXCR4的錶達,提示兩種化療藥物髮揮作用的機製可能涉及到CXCR4基因。
목적:관찰민감제량하배린선알(CTX)화필유비성(THP)대체외배양인횡문기육류RD세포주CXCR4기인표체적영향。방법체외배양RD세포주;MTT시험명학CTX화THP대RD세포적효응제량;세포화흔실험검측RD세포적천이능력;RT- PCR급Western blot법검측RD세포CXCR4표체。결과(1)민감제량하CTX(30 mmol/L)화THP(1000 ng/mL)균가억제RD세포적천이(P<0.05),량약연합응용대세포천이억제작용증강,교대조조차이유비상현저성(P<0.01);(2)각약물처리조(CTX,THP,CTX+THP)약물작용우RD세포24 h후CXCR4단백적표체교대조조감소,기차이균유현저성(P<0.05);기중CTX+THP조교CTX조CXCR4단백표체감소경명현(P<0.05),단여THP조비교,차이무현저성(P>0.05);(3)동양조건하,각약물처리조(CTX,THP,CTX+THP)여대조조비교,RD세포CXCR4 mRNA 적표체균감소,차이균유현저성(P<0.05);CTX+THP조교CTX조CXCR4 mRNA표체감소명현(P<0.05),단교THP조차이무현저성(P>0.05)。결론화료약물CTX화THP균능억제인횡문기육류RD세포적증식;민감제량하적량충약물균능억제RD세포적천이급하조RD세포CXCR4적표체,제시량충화료약물발휘작용적궤제가능섭급도CXCR4기인。
Objective To investigate the expression of chernokine CXC motif receptor 4 CXCR4 in human rhabdomyosarcoma RD cells,and its sensitivity to chemotherapeutic drugs CTX and THP,and to explore this chemokine receptor ’ s mechanism under sensitive dose about CTX/THP. Methods Human rhabdomyosarcoma RD cells were cultured in vitro.Dose-effects of chemotherapeutic drugs including CTX,THP on human rhabdomyosarcoma RD cells were investigated by MTT assay.The migratory ability was examined by wound healing assay.Expression of protein and mRNA of CXCR4 human rhabdomyosarcoma RD cells were detected by Western blotting and RT-PCR.Results CXCR4 expressed in human rhabdomyosarcoma RD cells.Dose-effects of chemotherapeutic drugs including CTX, THP are 30 mmol/L and 1000 ng/mL.Dose-effects of chemotherapeutic drugs including CTX,THP could inhibit rhabdomyosarcoma RD cells migration in vitro (P<0.05 ),synergistic effect (P<0.01 ) was found between two medicines.Chemotherapeutic drugs CTX,THP decreased (P <0.05 ) the expression of CXCR4 protein and mRNA level in human rhabdomyosarcoma RD cells,but no synergistic effect (P >0.05 ) was found between two medicines.Conclusions CTX and THP could inhibit human rhabdomyosarcoma RD cells migration in vitro and down-regulate the expression of chemokine receptor CXCR4, indicating that chemotherapy can inhibit the progression of tumor cells by down-regulating the expression of CXCR4.