CAJ | 학술논문

目的：观察环孢素A（ CsA)对创伤失血性休克大鼠的治疗作用。方法采用144只健康成年SD大鼠,分为正常对照组、休克对照组、乳酸林格氏液（ LR)复苏组、CsA 1 mg/kg、5 mg/kg和10 mg/kg组。建立创伤失血性休克模型后,观察不同剂量CsA治疗对休克动物的存活时间和24 h存活率的影响,同时通过股动脉和左心室插管测定血流动力学指标,观察CsA对大鼠平均动脉血压（ MAP)、左心室收缩压（ LVSP)、左心室舒张末压（ LVEDP)、左心室压力最大上升/下降速率（± dp/dtmax )和心率（ HR)的影响。结果 CsA（5 mg/kg和10 mg/kg)可明显提高创伤失血性休克大鼠的存活时间和24 h存活率。休克后大鼠血流动力学指标包括MAP、LVSP、± dp/dtmax和HR都显著降低,LR复苏可使各项指标有所回升,但显著低于正常对照组；CsA（5 mg/kg和10 mg/kg)可明显升高LVSP和± dp/dtmax ,在复苏2 h时明显高于LR组,并接近正常水平。结论 CsA显示出较好的抗创伤失血性休克作用,其中以5 mg/kg和10 mg/kg的给药剂量效果明显。
목적：관찰배포소A（ CsA)대창상실혈성휴극대서적치료작용。방법채용144지건강성년SD대서,분위정상대조조、휴극대조조、유산림격씨액（ LR)복소조、CsA 1 mg/kg、5 mg/kg화10 mg/kg조。건립창상실혈성휴극모형후,관찰불동제량CsA치료대휴극동물적존활시간화24 h존활솔적영향,동시통과고동맥화좌심실삽관측정혈류동역학지표,관찰CsA대대서평균동맥혈압（ MAP)、좌심실수축압（ LVSP)、좌심실서장말압（ LVEDP)、좌심실압력최대상승/하강속솔（± dp/dtmax )화심솔（ HR)적영향。결과 CsA（5 mg/kg화10 mg/kg)가명현제고창상실혈성휴극대서적존활시간화24 h존활솔。휴극후대서혈류동역학지표포괄MAP、LVSP、± dp/dtmax화HR도현저강저,LR복소가사각항지표유소회승,단현저저우정상대조조；CsA（5 mg/kg화10 mg/kg)가명현승고LVSP화± dp/dtmax ,재복소2 h시명현고우LR조,병접근정상수평。결론 CsA현시출교호적항창상실혈성휴극작용,기중이5 mg/kg화10 mg/kg적급약제량효과명현。
Objective To investigate the beneficial effects of cyclosporin A ( CsA) on traumatic hemorrhagic shock in rats. Methods The traumatic hemorrhagic shock model was adopted in 144 SD rats which were divided into 6 groups: sham-operated group,shock control group,lactated Ringer's solution ( LR) group,CsA 1 mg/kg,5 mg/kg and 10 mg/kg group. The effects of three doses of CsA on the animal survival time and 24 h survival rate were observed,and the effects of CsA on hemodynamic parameters,including mean arterial blood pressure ( MAP) ,left intraventricular systolic pressure ( LVSP) ,left ventricular end-diastolic pressure ( LVEDP) ,maximal change rate of left intraven-tricular pressure ( ± dp/dtmax ) and heart rate ( HR) were also observed. Results CsA at the concentration of 5 mg/kg and 10 mg/kg can significantly increase the survival time and 24 h survival rate of shock rats,the survival rate was increased to 56. 3% from 25% of LR group. After shock,the hemodynamic parameters were significantly decreased including MAP,LVSP and ± dp/dtmax ,LR infusion only improved the hemodynamics to some extent,which were significantly lower than those in sham-operated group. CsA (5 mg/kg and 10 mg/kg) can signifi-cantly improve the hemodynamics of shock rats including LVSP and ± dp/dtmax ,which were increased at 2 h after resuscitation as compared to LR group,and return to about normal levels. 1 mg/kg of CsA also restored the hemodynamic parameters, but there were no significant differences between CsA 1 mg/kg group and LR group. Conclusion CsA has good beneficial effect on traumatic hemorrhagic shock,and 5 mg/kg and 10 mg/kg of CsA showed a better effect.