CAJ | 학술논문

目的：探讨凋亡抑制蛋白Survivin在喉癌中的表达及其与细胞增殖的相关性。方法应用免疫组化（ SP）法和流式细胞术对63例喉鳞状细胞癌组织及15例正常喉组织进行Survivin蛋白检测和DNA含量分析。结果 Survivin在正常喉组织中不表达，在喉癌标本中阳性表达为45/63（71．4％），其阳性表达与分化程度及有无淋巴结转移有关（P＜0．05），而与患者年龄、性别、临床分期无关（P＞0．05）。喉癌的DNA指数（DI）和增殖指数（PI）均比喉正常组织高（P＜0．05），且PI值与Survivin阳性表达呈正相关（P＜0．05）。结论在喉癌中Survivin均有表达，其可能导致喉癌细胞过度增殖，影响喉癌的发生和发展，可能成为今后喉癌诊断和治疗的新靶点。
목적：탐토조망억제단백Survivin재후암중적표체급기여세포증식적상관성。방법응용면역조화（ SP）법화류식세포술대63례후린상세포암조직급15례정상후조직진행Survivin단백검측화DNA함량분석。결과 Survivin재정상후조직중불표체，재후암표본중양성표체위45/63（71．4％），기양성표체여분화정도급유무림파결전이유관（P＜0．05），이여환자년령、성별、림상분기무관（P＞0．05）。후암적DNA지수（DI）화증식지수（PI）균비후정상조직고（P＜0．05），차PI치여Survivin양성표체정정상관（P＜0．05）。결론재후암중Survivin균유표체，기가능도치후암세포과도증식，영향후암적발생화발전，가능성위금후후암진단화치료적신파점。
Objective The aim of this study is to investigate the expression of Survivin in laryngeal car-cinoma and to elucidate the relationship between cell proliferation and the expression of Survivin .Methods The expression and DNA quantification of Survivin in 63 cases of laryngeal carcinoma and 15 cases of normal laryngeal tissues were detected by immunohistochemical staining ( SP) and flow cytometry .Results Forty-five sections of laryngeal carcinoma were positive for survivin as determined by immunohistochemical staining ,but Survivin was undetected in normal laryngeal tissues .The intensity of Survivin expression was significantly increased with tumor differentiation and lymph node metastasis (P<0.05),and was negatively correlated with age ,gender and clinical stage of patients(P>0.05).High DNA index(DI)and proliferation index(PI)were detected in laryngeal carcino-ma(P<0.05),and PI was positively correlated with expression of Survivin (P<0.05).Conclusion Survivin overexpression triggers laryngeal carcinoma cell hyperproliferation ,especially in development of laryngeal carcino-ma.These data identify Survivin as an important target in laryngeal carcinoma and provide a translational pathway for developing new therapies against this target .