CAJ | 학술논문

目的：初步探索近红外荧光染料复合物PZ-1009在肿瘤成像中的应用价值。方法成功建立裸鼠肿瘤模型后，尾静脉注射复合物（10 nmoL/20 g），采用活体显像仪在不同时间点进行图像采集，在荧光强度最高时间点处死裸鼠，取肿瘤组织及重要器官成像。结果注射1h后肿瘤部位即出现强烈的荧光信号，采用图像软件测得6 h时信号强度最大，直至48 h肿瘤部位仍可见少量荧光信号，且肿瘤任何时间点的荧光信号强度都高于正常部位（ P＜0．05）；注射染料后6 h处死裸鼠，取肿瘤及主要器官进行显像，观察到除肠道外各器官都有较低荧光信号，但肿瘤仍为最强。结论近红外七甲川菁染料MHI-148与其他物质耦合后的新物质PZ-1009仍具有明显的肿瘤靶向性，为后期的多模态探针制备奠定了良好的基础。
목적：초보탐색근홍외형광염료복합물PZ-1009재종류성상중적응용개치。방법성공건립라서종류모형후，미정맥주사복합물（10 nmoL/20 g），채용활체현상의재불동시간점진행도상채집，재형광강도최고시간점처사라서，취종류조직급중요기관성상。결과주사1h후종류부위즉출현강렬적형광신호，채용도상연건측득6 h시신호강도최대，직지48 h종류부위잉가견소량형광신호，차종류임하시간점적형광신호강도도고우정상부위（ P＜0．05）；주사염료후6 h처사라서，취종류급주요기관진행현상，관찰도제장도외각기관도유교저형광신호，단종류잉위최강。결론근홍외칠갑천정염료MHI-148여기타물질우합후적신물질PZ-1009잉구유명현적종류파향성，위후기적다모태탐침제비전정료량호적기출。
Objective To assess the applicative value of near infrared fluorescence compound PZ -1009 in tumor imaging .Methods Near infrared fluorescence imaging properties of the compound was evaluated in a mouse model of ovarian cancer .The athymic nude mice were inoculated with SKOV -3 ovarian cancer cells and imaged in vivo and ex vivo after injecting PZ -1009 and were performed periodically .Results The tumor loca-tion was clearly imaged after 1 h of injection.The maximum fluorescence intensity was at 6 h time point and there was still a few fluorescent signal in tumors after 48hrs.A professional software was used to measure the intensity including all tumors and normal regions .The results showed that the intensity of tumors was higher than all normal regions in any time points .Conclusion The compound of PZ -1009 can efficiently target tumor sites and has great potential in noninvasive real time early tumor diagnosis .