CAJ | 학술논문

目的：研究血管生成素-2（Ang-2）与CD105在乳腺癌中的表达以及它们与乳腺癌发生发展的关系。方法收集乳腺癌组织30例、癌前病变的乳管内乳头状瘤组织15例以及正常乳腺组织15例，采用免疫组化、Western blot和RT-PCR三种技术手段，检测Ang-2和CD105在正常乳腺组织、癌变前乳腺组织以及癌变乳腺组织中的表达情况；探讨Ang-2、CD105与乳腺癌临床病理学特征的关系。结果乳腺癌癌组织、癌变前乳腺组织中CD105和Ang-2蛋白与mRNA表达水平均比正常组织显著升高；且正常乳腺组织、癌变前乳腺组织到癌变的乳腺组织，CD105和Ang-2蛋白、mRNA水平呈逐步上升的趋势；CD105和Ang-2的蛋白与mRNA表达水平均与乳腺癌肿瘤大小、肿瘤浸润、淋巴结转移呈正相关，与乳腺癌分化程度呈负相关性。结论 CD105和Ang-2参与了乳腺癌的发生与发展，对乳腺癌的早期诊断和预后判断具有一定的临床应用价值。
목적：연구혈관생성소-2（Ang-2）여CD105재유선암중적표체이급타문여유선암발생발전적관계。방법수집유선암조직30례、암전병변적유관내유두상류조직15례이급정상유선조직15례，채용면역조화、Western blot화RT-PCR삼충기술수단，검측Ang-2화CD105재정상유선조직、암변전유선조직이급암변유선조직중적표체정황；탐토Ang-2、CD105여유선암림상병이학특정적관계。결과유선암암조직、암변전유선조직중CD105화Ang-2단백여mRNA표체수평균비정상조직현저승고；차정상유선조직、암변전유선조직도암변적유선조직，CD105화Ang-2단백、mRNA수평정축보상승적추세；CD105화Ang-2적단백여mRNA표체수평균여유선암종류대소、종류침윤、림파결전이정정상관，여유선암분화정도정부상관성。결론 CD105화Ang-2삼여료유선암적발생여발전，대유선암적조기진단화예후판단구유일정적림상응용개치。
Objective To explore the expression of Ang -2 and CD105 in breast cancer and their po-tential associations with the cancerization and progression of breast cancer .Methods Thirty patients with breast cancer ( cancerous tissue ) ,15 intraductal papilloma cases ( precancer tissue ) and 15 normal breast tissue were col-lected for each group ,respectively .Immunohistochemistry ,western blot and RT-PCR techniques were used to ex-amine the expressions of Ang -2 and CD105 in cancerous breast tissue ,precancer breast tissue and normal breast tissue at both protein and mRNA levels .The possible correlations of Ang -2 and CD105 expression with clinico-pathological characteristics of the breast cancer were analyzed .Results The expressions of CD105 and Ang-2 in cancer and precancer tissues were higher than in normal tissues at both protein and mRNA levels ,and the trend of their expressions was increased from normal to precancer ,and cancer tissue .The expressions of CD 105 and Ang-2 protein and mRNA were positively correlated with tumor size ,invasion,lymph node metastasis,and negatively correlated with the differentiation of the cancer .Conclusion CD105 and Ang-2 are involved in the canceriza-tion and pregression of breast cancer .These two biomarkers can serve as two useful indicators in assisting diagno-sis and prognosis of breast cancer .