牡丹江医学院学报
牡丹江醫學院學報
모단강의학원학보
JOURNAL OF MUDANJIANG MEDICAL COLLEGE
2014年
3期
1-4
,共4页
贾桦%王莹%张莲香%马全瑞%佟雷%佟晓杰
賈樺%王瑩%張蓮香%馬全瑞%佟雷%佟曉傑
가화%왕형%장련향%마전서%동뢰%동효걸
脱细胞异种神经移植体%BMSCs%移植%神经再生
脫細胞異種神經移植體%BMSCs%移植%神經再生
탈세포이충신경이식체%BMSCs%이식%신경재생
Acellular nerve xenograft%Bone marrow stromal cells%Transplantation%Nerve regeneration
目的:对脱细胞异种神经的生物相容性进行评价,证明应用其复合BMSCs促进神经再生和功能恢复的效果。方法:采用化学萃取法制备兔的脱细胞神经,应用扫描电镜观察其超微结构;将BMSCs种植到神经支架构建神经移植体,体外培养后观察细胞形态;用构建的异种神经移植体桥接大鼠坐骨神经缺损,术后8周,检测胫前肌湿重比率,对再生神经进行组织形态学分析;并应用免疫荧光染色检测神经内NGF的表达。结果:脱细胞异种神经较完整地保留了天然立体的施万细胞基底膜管结构,完全地移除了神经内的施万细胞、轴突和髓鞘成分;在体外培养中支持种植的BMSCs黏附和生长。神经移植术后,与单纯异种脱细胞神经移植组相比,复合BMSCs的异种神经移植体移植能使胫前肌湿重比率增加、有髓神经纤维数目、髓鞘厚度和轴突直径增加;增加再生神经内NGF的表达。结论:异种脱细胞神经移植体具备天然的立体结构,移除了施万细胞、髓鞘等抗原成分;神经支架无细胞毒性,具有良好的细胞相容性;复合BMSCs的异种神经移植体可显著地促进神经再生及功能恢复。
目的:對脫細胞異種神經的生物相容性進行評價,證明應用其複閤BMSCs促進神經再生和功能恢複的效果。方法:採用化學萃取法製備兔的脫細胞神經,應用掃描電鏡觀察其超微結構;將BMSCs種植到神經支架構建神經移植體,體外培養後觀察細胞形態;用構建的異種神經移植體橋接大鼠坐骨神經缺損,術後8週,檢測脛前肌濕重比率,對再生神經進行組織形態學分析;併應用免疫熒光染色檢測神經內NGF的錶達。結果:脫細胞異種神經較完整地保留瞭天然立體的施萬細胞基底膜管結構,完全地移除瞭神經內的施萬細胞、軸突和髓鞘成分;在體外培養中支持種植的BMSCs黏附和生長。神經移植術後,與單純異種脫細胞神經移植組相比,複閤BMSCs的異種神經移植體移植能使脛前肌濕重比率增加、有髓神經纖維數目、髓鞘厚度和軸突直徑增加;增加再生神經內NGF的錶達。結論:異種脫細胞神經移植體具備天然的立體結構,移除瞭施萬細胞、髓鞘等抗原成分;神經支架無細胞毒性,具有良好的細胞相容性;複閤BMSCs的異種神經移植體可顯著地促進神經再生及功能恢複。
목적:대탈세포이충신경적생물상용성진행평개,증명응용기복합BMSCs촉진신경재생화공능회복적효과。방법:채용화학췌취법제비토적탈세포신경,응용소묘전경관찰기초미결구;장BMSCs충식도신경지가구건신경이식체,체외배양후관찰세포형태;용구건적이충신경이식체교접대서좌골신경결손,술후8주,검측경전기습중비솔,대재생신경진행조직형태학분석;병응용면역형광염색검측신경내NGF적표체。결과:탈세포이충신경교완정지보류료천연입체적시만세포기저막관결구,완전지이제료신경내적시만세포、축돌화수초성분;재체외배양중지지충식적BMSCs점부화생장。신경이식술후,여단순이충탈세포신경이식조상비,복합BMSCs적이충신경이식체이식능사경전기습중비솔증가、유수신경섬유수목、수초후도화축돌직경증가;증가재생신경내NGF적표체。결론:이충탈세포신경이식체구비천연적입체결구,이제료시만세포、수초등항원성분;신경지가무세포독성,구유량호적세포상용성;복합BMSCs적이충신경이식체가현저지촉진신경재생급공능회복。
Objective:To evaluate the biocompatibility of acellular nerve xenografts , after bridging nerve gaps by utilizing the BM-SCs-combined xenogenic nerve grafts , the effectiveness of axonal regeneration and functional restoration was demonstrated .Methods:The acellular nerves derived from rabbits through chemically extracted processing were prepared and the ultrastructural organization was subsequently observed using the SEM .The nerve scaffolds were also seeded with BMSCs to construct tissue engineered transplants , af-ter culture in vitro , the ultrastructure of the integrated nerve grafts was observed .And then the xenogenic nerve grafts were employed to bridge sciatic nerve gaps in rats .After 8 weeks post-transplantation , the weight ratios of tibialis anterior muscle were detected , be-sides, the morphological analysis was conducted , and the expression of NGF in the regenerated nerves was carried out via immunostain -ing.Results:The tridimensional architecture of basal lamina tube in extracellular matrix was preserved intactly , and the Schwann cells, axons as well as myelin sheath were removed completely in the acellular nerves .In vitro the nerve grafts exhibited the effects on supporting cells adhesion and growth .After nerve grafting , compared with acellular nerve blank control group , the xenogenic nerve transplantation integrating with BMSCs increased the weight ratios of tibialis anterior muscle , myelinated fiber number , axon diameter and myelin sheath thickness .Moreover, the expression of NGF in regenerated nerves is elevated apparently .Conclusion: The tridi-mensional architecture of basal lamina tube in extracellular matrix is preserved intactly , and the Schwann cells as well as myelin sheath are removed completely in the acellular nerve xenografts .The acellular nerve grafts do not show cytotoxicity , but possess favourable cell compatibility.Nerve regeneration and functional rehabilitation are advanced significantly for xenogenic nerve transplantation integrating with BMSCs .