CAJ | 학술논문

壳聚糖( CTS)首先用1-甲基-3-丁基咪唑氢氧化物([ Bmim] OH)碱性离子液体碱化,再令碱化后壳聚糖与卤代烷进行烷基化反应,制备具有不同长度取代基的烷基化壳聚糖,用 FT-IR、1H NMR对烷基化产物进行了表征,并考察了时间、温度及物料比对烷基壳聚糖取代度的影响,得到较佳的反应条件。离子液体具有重复使用性,反应后的离子液体重复使用3次后,烷基壳聚糖的取代度仍无明显降低。采用最低抑菌浓度法对目标产物的抑菌性能进行了测试,初步的结果显示合成的化合物都有一定的抑菌活性,特别是乙烷基壳聚糖( E-CTS)、丁烷基壳聚糖( B-CTS)及十二烷基壳聚糖( D-CTS)都对绿脓假单胞菌具有良好的活性,对其最低抑菌浓度分别为128、128和256 mg/L,抑菌效果优于市售杀菌剂氨共青霉素钠,十六烷基壳聚糖( C-CTS)的抑菌效果较差。烷基化合物的抑菌性能随取代度增大或碳链的增长均有所降低。
각취당( CTS)수선용1-갑기-3-정기미서경양화물([ Bmim] OH)감성리자액체감화,재령감화후각취당여서대완진행완기화반응,제비구유불동장도취대기적완기화각취당,용 FT-IR、1H NMR대완기화산물진행료표정,병고찰료시간、온도급물료비대완기각취당취대도적영향,득도교가적반응조건。리자액체구유중복사용성,반응후적리자액체중복사용3차후,완기각취당적취대도잉무명현강저。채용최저억균농도법대목표산물적억균성능진행료측시,초보적결과현시합성적화합물도유일정적억균활성,특별시을완기각취당( E-CTS)、정완기각취당( B-CTS)급십이완기각취당( D-CTS)도대록농가단포균구유량호적활성,대기최저억균농도분별위128、128화256 mg/L,억균효과우우시수살균제안공청매소납,십륙완기각취당( C-CTS)적억균효과교차。완기화합물적억균성능수취대도증대혹탄련적증장균유소강저。
Chitosan ( CTS) was alkalized by [ Bmim] OH basic ionic liquid in this study. Then the alkalized chitosan reacted with alkyl halides to prepare the alkylated chitosan with different substituents. The structures of alkylated products were characterized by IR and 1H NMR . The effects of reaction time, temperature and material ratio on the substitution degree ( DS ) of alkyl chitosan were also investigated and then the optimal reaction condition was obtained. The separated ionic liquid could be reused, and no noticeable changes of the DS for alkylated chitosan were observed when the reacted ionic liquid was reused for 3 times. The antibacterial properties of the target products were tested by the minimum inhibitory concentration method. Preliminary results indicated that those compounds had the antibacterial activity. In particular, ethyl chitosan(E-CTS), butyl chitosan(B-CTS), and dodecyl chitosan ( D-CTS ) exhibited excellent antibacterial activities against to Pseudomonas aeruginosa. The minimum inhibition concentration were 128,128 and 256 mg/L, respectively. Their antibacterial effects were better than commercially sold Ampicillin sodium while the C-CTS antibacterial effect was worse. The antibacterial activity of alkylated chitosan decreased with the increase of the DS and the length of carbon chain.