CAJ | 학술논문

目的：通过体外实验对新型生物吸收性多孔碳酸化羟基磷灰石（CAP）支架材料的骨传导性进行评价。方法：体外分离培养大鼠骨髓间充质干细胞，向成骨诱导后，定植于不同孔隙率及不同碳酸根含量的CAP支架材料上共同培养，通过扫描电镜、细胞黏附及增殖检测（MTT法）、碱性磷酸酶（ALP）定量检测、骨钙素（OCN）定量检测，评价成骨细胞在支架材料上的附着、增殖和分化情况。结果：成骨细胞定植于不同孔隙率及碳酸根含量的支架材料上，4 h均已开始黏附、且增殖情况良好。分化实验中，ALP和OCN在各组支架材料分化良好。不同孔隙率支架材料组间比较，40%孔隙率的实验组对ALP分化的促进作用有明显优势。结论：CAP支架材料有良好的生物相容性和骨传导性，是一种良好的组织工程支架材料。
목적：통과체외실험대신형생물흡수성다공탄산화간기린회석（CAP）지가재료적골전도성진행평개。방법：체외분리배양대서골수간충질간세포，향성골유도후，정식우불동공극솔급불동탄산근함량적CAP지가재료상공동배양，통과소묘전경、세포점부급증식검측（MTT법）、감성린산매（ALP）정량검측、골개소（OCN）정량검측，평개성골세포재지가재료상적부착、증식화분화정황。결과：성골세포정식우불동공극솔급탄산근함량적지가재료상，4 h균이개시점부、차증식정황량호。분화실험중，ALP화OCN재각조지가재료분화량호。불동공극솔지가재료조간비교，40%공극솔적실험조대ALP분화적촉진작용유명현우세。결론：CAP지가재료유량호적생물상용성화골전도성，시일충량호적조직공정지가재료。
Objective: To evaluate the osteoconductivity of a novel porous carbonated apitite (CAP) in vitro. Methods:Rat bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured. Well growing BMSCs were cultured with culture medium containing osteogenic inductor. Osteoblast cells obtained by osteogenic induction were seeded on the surfaces of CAP scaffolds with different porosities and carbonate contents. SEM, MTT analysis, alkaline phosphatase (ALP) activity assay, osteocalcin (OCN) activity assay were carried out to measure the osteogenic potentials of the BMSCs cells. Results: BMSCs cells could well attach to and extend on all experiment and control group samples. The alkaline phosphatase (ALP) activity assay, osteocalcin activity assay showed that BMSCs cells differentiated well on all CAP groups. And 40% NaCl content groups were more outstanding in ALP activity assay. Conclusion: The porous CAP is biocompatible and osteoconductible.