口腔颌面外科杂志
口腔頜麵外科雜誌
구강합면외과잡지
CHINESE JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
2014年
3期
170-174
,共5页
降钙素基因相关肽%上颌窦底提升术%成骨诱导%骨髓间充质干细胞%犬
降鈣素基因相關肽%上頜竇底提升術%成骨誘導%骨髓間充質榦細胞%犬
강개소기인상관태%상합두저제승술%성골유도%골수간충질간세포%견
calcitonin gene-related peptide%maxillary sinus augmentation%osteogenic induction%bone marrow mesenchymal stem cells%dog
目的：观察降钙素基因相关肽（calcitonin gene-related peptide，CGRP）在犬骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）向成骨细胞分化过程中，及应用BMSCs构建组织工程骨提升犬上颌窦底后骨组织中的表达情况。方法：体外分离培养犬BMSCs，通过成骨诱导采用实时荧光定量PCR（real-time quantitative polymerase chain reaction，Q-PCR）和免疫荧光技术检测CGRP在不同时间点的表达情况。犬上颌窦底提升术后，分别在两侧上颌窦底植入BMSCs+Bio-Oss骨粉（实验组）和Bio-Oss骨粉（对照组）。12周后处死并获取动物标本，对窦底区骨组织行CGRP免疫组化染色。结果：体外实验示CGRP表达量随成骨诱导分化呈时间依赖性递增（P<0.05）；上颌窦底提升实验组CGRP表达量明显高于对照组（P<0.05），且CGRP主要分布在骨再生代谢活跃区域。结论：CGRP表达量与骨再生过程密切相关，可考虑作为骨代谢活跃度的标志。
目的：觀察降鈣素基因相關肽（calcitonin gene-related peptide，CGRP）在犬骨髓間充質榦細胞（bone marrow mesenchymal stem cells，BMSCs）嚮成骨細胞分化過程中，及應用BMSCs構建組織工程骨提升犬上頜竇底後骨組織中的錶達情況。方法：體外分離培養犬BMSCs，通過成骨誘導採用實時熒光定量PCR（real-time quantitative polymerase chain reaction，Q-PCR）和免疫熒光技術檢測CGRP在不同時間點的錶達情況。犬上頜竇底提升術後，分彆在兩側上頜竇底植入BMSCs+Bio-Oss骨粉（實驗組）和Bio-Oss骨粉（對照組）。12週後處死併穫取動物標本，對竇底區骨組織行CGRP免疫組化染色。結果：體外實驗示CGRP錶達量隨成骨誘導分化呈時間依賴性遞增（P<0.05）；上頜竇底提升實驗組CGRP錶達量明顯高于對照組（P<0.05），且CGRP主要分佈在骨再生代謝活躍區域。結論：CGRP錶達量與骨再生過程密切相關，可攷慮作為骨代謝活躍度的標誌。
목적：관찰강개소기인상관태（calcitonin gene-related peptide，CGRP）재견골수간충질간세포（bone marrow mesenchymal stem cells，BMSCs）향성골세포분화과정중，급응용BMSCs구건조직공정골제승견상합두저후골조직중적표체정황。방법：체외분리배양견BMSCs，통과성골유도채용실시형광정량PCR（real-time quantitative polymerase chain reaction，Q-PCR）화면역형광기술검측CGRP재불동시간점적표체정황。견상합두저제승술후，분별재량측상합두저식입BMSCs+Bio-Oss골분（실험조）화Bio-Oss골분（대조조）。12주후처사병획취동물표본，대두저구골조직행CGRP면역조화염색。결과：체외실험시CGRP표체량수성골유도분화정시간의뢰성체증（P<0.05）；상합두저제승실험조CGRP표체량명현고우대조조（P<0.05），차CGRP주요분포재골재생대사활약구역。결론：CGRP표체량여골재생과정밀절상관，가고필작위골대사활약도적표지。
Objective: To observe the expressive levels of calcitonin gene-related peptide (CGRP) in bone marrow mesenchymal stem cells (BMSCs) during osteoinduction, and in the remodeled bone of maxillary sinus floor augmentation. Methods:In vitro, the BMSCs were isolated from Beagle dogs and were classified into osteogenic-induced group and non-induced group. Expression of the CGRP was detected by Q-PCR and immunofluorescence in the different time-points. In vivo, both right and left maxillary sinus of three healthy adult dogs were divided into experimental group and control group randomly. BMSCs complexed with Bio-Oss granules were applied for canine maxillary sinus augmentation models, while Bio-Oss granules alone served as controls. Immunohistochemical analysis were performed after 12 weeks on these bone substitutes. Results: Q-PCR and immunofluorescence examinations demonstrated the expression of CGRP in osteogenic-induced group was increased in a time-dependent manner. After maxillary sinus floor elevation, the expressive levels of CGRP in experimental group were superior than that of control group (P<0.05). CGRP was mainly distributed in the region where osteogenesis metabolism was active. Conclusion: The expression of CGRP was related to the progress of bone regeneration, and it can be regarded as an indicator of the activity of osteogenesis metabolism.
