CAJ | 학술논문

以水晶葡萄盆栽苗为试验材料，研究不同质量浓度的秋水仙碱，在不同处理时间下，运用套罩法进行染色体加倍诱导，确定最佳处理质量浓度和处理时间，并对诱导的变异植株与对照植株进行形态学、生理学、解剖学和细胞学鉴定。结果表明：质量浓度为8 mg/mL的秋水仙碱处理5 d效果最佳，处理后的植株为变异四倍体的可能性较大；经该处理后的植株在节间距、叶片厚度、SOD酶活性、气孔密度及大小、导管直径及长短、细胞大小等指标上均明显强于对照处理。
이수정포도분재묘위시험재료，연구불동질량농도적추수선감，재불동처리시간하，운용투조법진행염색체가배유도，학정최가처리질량농도화처리시간，병대유도적변이식주여대조식주진행형태학、생이학、해부학화세포학감정。결과표명：질량농도위8 mg/mL적추수선감처리5 d효과최가，처리후적식주위변이사배체적가능성교대；경해처리후적식주재절간거、협편후도、SOD매활성、기공밀도급대소、도관직경급장단、세포대소등지표상균명현강우대조처리。
Potting Shuijing grapes were used as materials.The effects of colchicines treatment at different time and concentrations were studied on polyploid induction.The treated parts of the plants were shrouded by absorbent cotton to ensure the optimal treating time and concentration.Comparisons of configurations,physiology and biochemistry,anatomy,cytology identification were done between the variant plants and contrast plants.The results showed that the best induction rate was achieved at 8 mg/mL of colchicines treatment for 5 days.The treated plants showed the great possibility of polyploid and obviously stronger indications than water treatment plants in the distance between nodes,leaf thickness,SOD enzyme activity,stomatal density and size,tube diameter and length,and cell size.