CAJ | 학술논문

肠炎沙门氏菌(Salmonella enterica serovar Enteritidis)能引起畜禽的胃肠炎以及食物中毒，其血清型鉴定较为复杂。为了挖掘肠炎沙门氏菌血清型特异性基因，以44个已测序沙门氏菌基因组构建数据库，利用肠炎沙门氏菌所有蛋白编码序列为查询序列，通过BLASTN比对，挖掘出6个肠炎沙门氏菌血清型特异性基因（SEN1382, SEN1383, SEN1388, SEN1936, SEN1945和SEN1959），其中4个基因编码噬菌体相关蛋白。同时，研究分析了肠炎沙门氏菌血清型特异性基因的理化性质、二级结构、三级结构、亚细胞定位、信号肽和跨膜区等特征，结果表明肠炎沙门氏菌血清型特异性基因具有多样性特征。挖掘的血清型特异性基因可为鉴定肠炎沙门氏菌提供检测靶标。
장염사문씨균(Salmonella enterica serovar Enteritidis)능인기축금적위장염이급식물중독，기혈청형감정교위복잡。위료알굴장염사문씨균혈청형특이성기인，이44개이측서사문씨균기인조구건수거고，이용장염사문씨균소유단백편마서렬위사순서렬，통과BLASTN비대，알굴출6개장염사문씨균혈청형특이성기인（SEN1382, SEN1383, SEN1388, SEN1936, SEN1945화SEN1959），기중4개기인편마서균체상관단백。동시，연구분석료장염사문씨균혈청형특이성기인적이화성질、이급결구、삼급결구、아세포정위、신호태화과막구등특정，결과표명장염사문씨균혈청형특이성기인구유다양성특정。알굴적혈청형특이성기인가위감정장염사문씨균제공검측파표。
Salmonella enterica serovar Enteritidis is one of the major causes of gastroenteritis and food poisoning in humans due to the consumption of poultry derivatives. To mine serovar-specific genes of S. enterica serovar Enteritidis, all CDSs of it were searched in a searching database of 44 other Salmonella genomic sequences using SMM-system tool. Six serovar-specific genes were identified including SEN1382, SEN1383, SEN1388, SEN1936, SEN1945, and SEN1959. Among them, four genes decoded phage-related proteins. The characteristics of these genes were analyzed including physicochemical properties, secondary structure, three-dimensional structure, signal peptide, transmembrane regions, and subcellular localization. The results indicated the diversification of these genes. These serovar-specific genes can be used as the targets for detecting and identifying S. enterica serovar Enteritidis.