CAJ | 학술논문

为明确辽宁省和安徽省玉米弯孢叶斑病菌的生理分化和遗传多样性及其近缘关系。利用UP-PCR、ISSR分子标记方法,对采集于辽宁省和安徽省30株玉米弯孢叶斑病菌进行了遗传多样性分析。从供试引物中筛选获得具有多态性好且稳定的UP-PCR引物4个、ISSR引物12个,分别扩增出27条谱带和76条谱带,多态性条带比率分别为77.78%和89.47%。聚类分析结果表明,UP-PCR阈值在0.662处菌株被分为7个类群,ISSR阈值在0.681处菌株被分为8个类群,玉米弯孢叶斑病菌存在丰富的遗传变异,与地理来源无明显相关性。从稳定性、可操作性和多态性水平来看,ISSR技术更适合于分析玉米弯孢叶斑病菌的遗传多样性。
위명학요녕성화안휘성옥미만포협반병균적생리분화화유전다양성급기근연관계。이용UP-PCR、ISSR분자표기방법,대채집우요녕성화안휘성30주옥미만포협반병균진행료유전다양성분석。종공시인물중사선획득구유다태성호차은정적UP-PCR인물4개、ISSR인물12개,분별확증출27조보대화76조보대,다태성조대비솔분별위77.78%화89.47%。취류분석결과표명,UP-PCR역치재0.662처균주피분위7개류군,ISSR역치재0.681처균주피분위8개류군,옥미만포협반병균존재봉부적유전변이,여지리래원무명현상관성。종은정성、가조작성화다태성수평래간,ISSR기술경괄합우분석옥미만포협반병균적유전다양성。
In order to understand the physiological differentiation,the genetic diversity and the relationship of Curvularia lunata in Liaoning Province and Anhui Province. Genetic diversity of 30 isolates of Curvularia lunata from the major corn producing areas of Liaoning Province and Anhui Province in China was analyzed by UP-PCR ( Universally primed PCR) and ISSR( Internal simple sequence repeat) . The results showed that 4 primers of UP-PCR,12 primers of ISSR Genetic 27 and 76 polymorphic bands,accounted for 77. 78% and 89. 47% of total bands respectively. These molecular markers revealed the genetic diversity of the strains tested,and there was no signifi-cant correlation between variation and region . The correlation between UP-PCR and ISSR,the dendrogram based on UP-CR results revealed that the 30 isolates were clustered into 7 groups at the threshold of genetic similar coefficient of 0. 662,while ISSR were clustered into 8 groups at the threshold of genetic similar coefficient of 0. 681. The ISSR technique was suitable for the genetic diversity analysis of C. lunata in view of the level of stability,operability and polymorphism.