华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
3期
221-226
,共6页
范方军%王芳权%刘永峰%王军%朱金燕%李文奇%仲维功%杨杰
範方軍%王芳權%劉永峰%王軍%硃金燕%李文奇%仲維功%楊傑
범방군%왕방권%류영봉%왕군%주금연%리문기%중유공%양걸
水稻%稻瘟病%功能标记
水稻%稻瘟病%功能標記
수도%도온병%공능표기
Rice%Rice panicle blast%Functional markers
利用水稻稻瘟病抗病基因Pi-b、Pi-ta、Pikm和Pi54的功能标记检测2012年江苏省迟熟中粳稻预试64份品系,结合水稻穗颈瘟抗性鉴定,解析稻瘟病抗病基因Pi-b、Pi-ta、Pikm和Pi54在江苏省粳稻稻瘟病抗性育种中的作用。结果表明,在64份材料中含稻瘟病基因Pi-b的有49份,含Pi-ta的26份,含Pikm的19份,含Pi54的25份。64份品系的人工或病圃鉴定其抗性表现为2级中抗的有10份,3级感病的有43份,4级高感的有11份。64份品系中含有抗稻瘟病基因Pi-ta(26个新品系),或Pi-b、Pikm和Pi54组合(4个新品系),则该品系穗颈瘟抗性水平在2级中抗或3级感病,无4级高感,其他抗病基因组合其穗颈瘟抗性水平一般都存在4级高感。相关性分析表明,只有抗稻瘟病基因Pi-ta与穗颈瘟抗性存在显著相关性(r=0.344,P<0.01)。
利用水稻稻瘟病抗病基因Pi-b、Pi-ta、Pikm和Pi54的功能標記檢測2012年江囌省遲熟中粳稻預試64份品繫,結閤水稻穗頸瘟抗性鑒定,解析稻瘟病抗病基因Pi-b、Pi-ta、Pikm和Pi54在江囌省粳稻稻瘟病抗性育種中的作用。結果錶明,在64份材料中含稻瘟病基因Pi-b的有49份,含Pi-ta的26份,含Pikm的19份,含Pi54的25份。64份品繫的人工或病圃鑒定其抗性錶現為2級中抗的有10份,3級感病的有43份,4級高感的有11份。64份品繫中含有抗稻瘟病基因Pi-ta(26箇新品繫),或Pi-b、Pikm和Pi54組閤(4箇新品繫),則該品繫穗頸瘟抗性水平在2級中抗或3級感病,無4級高感,其他抗病基因組閤其穗頸瘟抗性水平一般都存在4級高感。相關性分析錶明,隻有抗稻瘟病基因Pi-ta與穗頸瘟抗性存在顯著相關性(r=0.344,P<0.01)。
이용수도도온병항병기인Pi-b、Pi-ta、Pikm화Pi54적공능표기검측2012년강소성지숙중갱도예시64빈품계,결합수도수경온항성감정,해석도온병항병기인Pi-b、Pi-ta、Pikm화Pi54재강소성갱도도온병항성육충중적작용。결과표명,재64빈재료중함도온병기인Pi-b적유49빈,함Pi-ta적26빈,함Pikm적19빈,함Pi54적25빈。64빈품계적인공혹병포감정기항성표현위2급중항적유10빈,3급감병적유43빈,4급고감적유11빈。64빈품계중함유항도온병기인Pi-ta(26개신품계),혹Pi-b、Pikm화Pi54조합(4개신품계),칙해품계수경온항성수평재2급중항혹3급감병,무4급고감,기타항병기인조합기수경온항성수평일반도존재4급고감。상관성분석표명,지유항도온병기인Pi-ta여수경온항성존재현저상관성(r=0.344,P<0.01)。
In this study,64 late maturity japonica test lines of Jiangsu Province were detected with the function-al markers of rice blast resistance genes Pi-b,Pi-ta,Pikm and Pi54 in rice. Combined with the identification of rice panicle blast resistance,the role of blast resistance genes Pi-b,Pi-ta,Pikm and Pi54 for Japonica rice breeding with blast resistance in Jangsu Province has been analysized. There were 49 lines containing rice blast resistance gene Pi-b,26 lines containing Pi-ta,19 lines containing Pikm,25 lines containing Pi54 in 64 lines. Through artificial or dis-ease nursery identification,the resistance performance into 2 levels( Moderate resistance) of disease resistance were 10 lines,3 levels( Susceptible) were 43 lines,4 levels( High susceptible) were 11 lines. If new lines contain blast resistance genes Pi-ta(26 new lines),or combined with Pi-b,Pikm and Pi54(4 new lines),the levels of panicle blast resistance showed 2 levels( Moderate resistance) or 3 levels( Susceptible) and never 4 levels( High suscepti-ble) ,while lines with other resistance gene combinations generally exhibited 4 grade levels of panicle blast resist-ance( High susceptible) . A correlation showed that only the rice blast resistance gene Pi-ta has certain correlation with Panicle Blast Resistance(r=0. 344,P<0. 01).