CAJ | 학술논문

通过人工气候箱处理、半薄切片观察、田间育性调查以及SSR分子标记技术,研究了甘蓝型油菜温敏雄性不育材料Huiyou50S的温度响应规律、细胞学败育特征、遗传模式及不育基因遗传定位。结果表明：Huiyou50S属于反型温敏不育系,低温不育、高温可育,育性转换临界温度接近昼24℃/夜14℃；Huiyou50S的花药败育发生在单核期,小孢子细胞内容物收缩、解体,最终只剩空壳,绒毡层细胞在单核后期提前降解消失；Huiyou50S ×中双11正反交F2、BC1的育性分离比符合1对隐性基因控制的遗传模式；采用分离群体分组分析法( BSA法)筛选到与不育基因连锁的SSR分子标记3个,并将不育基因初步定位在A10连锁群上。这些结果表明,Huiyou50S是一种反温敏型隐性细胞核不育系,可作为一种良好的研究温度诱导生理效应的生物模型,而且可以和Polima细胞质雄性不育、化学杀雄结合构建育性稳定的双重雄性不育。
통과인공기후상처리、반박절편관찰、전간육성조사이급SSR분자표기기술,연구료감람형유채온민웅성불육재료Huiyou50S적온도향응규률、세포학패육특정、유전모식급불육기인유전정위。결과표명：Huiyou50S속우반형온민불육계,저온불육、고온가육,육성전환림계온도접근주24℃/야14℃；Huiyou50S적화약패육발생재단핵기,소포자세포내용물수축、해체,최종지잉공각,융전층세포재단핵후기제전강해소실；Huiyou50S ×중쌍11정반교F2、BC1적육성분리비부합1대은성기인공제적유전모식；채용분리군체분조분석법( BSA법)사선도여불육기인련쇄적SSR분자표기3개,병장불육기인초보정위재A10련쇄군상。저사결과표명,Huiyou50S시일충반온민형은성세포핵불육계,가작위일충량호적연구온도유도생리효응적생물모형,이차가이화Polima세포질웅성불육、화학살웅결합구건육성은정적쌍중웅성불육。
At this study,a thermo-sensitive male sterile line in Brassica napus,named Huiyou50S,was used for research material:experimented with artificial climate chamber test,cytological observation,field fertility survey,and screening of SSR molecular markers. The results showed:Huiyou50S was a reverse thermo-sensitive genic male ster-ile line,it was male sterile under the condition of low temperature,but fertile under the high temperature condition, the stagnation temperature closed to 24 ℃( day )/14 ℃( night ) . The male sterility occoured at the uni-nucleate stage of microspores. Cytoplasm of the microspores was disintegrated and become an empty cell at later time. Mean-while,the tapetum disappeared rapidly at later period of uni-nucleate stage. The male sterility of Huiyou50S was controlled by single-locus recessive gene. By means of Bulked Segregation Analysis(BSA),we have found 3 SSR molecular markers linked with the male sterility gene of Huiyou50S,which were all located on A10 linkage group of B. napus genome. These results indicated that Huiyou50S is a novel type of reverse thermo-sensitive genic male ster-ile line. It can be used as a biological model for study physiological effect of temperature. Furthermore,it can com-bine with chemical gametocide or Polima cytoplasm male sterility to construct steady male sterility system.