CAJ | 학술논문

采用IRAP分子标记方法,设计引物对36份小麦材料进行PCR扩增,得到多态性丰富的指纹图谱,并进行聚类分析。结果显示：扩增出清晰稳定条带246条,片段大小多集中在250~2000 bp,多态性位点总计13个,各个品种扩增的位点数为0~12个,聚类分析结果能够反映36份小麦材料抗性有无以及强弱关系,表明IRAP分子标记适合应用于小麦抗旱品种筛选的研究分析。
채용IRAP분자표기방법,설계인물대36빈소맥재료진행PCR확증,득도다태성봉부적지문도보,병진행취류분석。결과현시：확증출청석은정조대246조,편단대소다집중재250~2000 bp,다태성위점총계13개,각개품충확증적위점수위0~12개,취류분석결과능구반영36빈소맥재료항성유무이급강약관계,표명IRAP분자표기괄합응용우소맥항한품충사선적연구분석。
Using IRAP molecular method,design primers and 36 wheat varieties were used for PCR amplifica-tion,polymorphic finger maps were obtained and clustering analysis. The results were as follows:246 bands were produced by IRAP,the range of amplification fragments were 250-2 000 bp,a total of 13 polymorphic loci,the range of different varieties was 0-12 . the results of clustering analysis can be clearly differentiate drought resistance be-tween 36 wheat varieties. The study showed that IRAP were suitable for screening drought resistance varieties in wheat.