传染病信息
傳染病信息
전염병신식
INFECTIOUS DISEASE INFORMATION
2014年
3期
152-156
,共5页
鲍春梅%宋新爱%崔恩博%王欢%张鞠玲%陈素明%张成龙%贾天野%曲芬%毛远丽
鮑春梅%宋新愛%崔恩博%王歡%張鞠玲%陳素明%張成龍%賈天野%麯芬%毛遠麗
포춘매%송신애%최은박%왕환%장국령%진소명%장성룡%가천야%곡분%모원려
志贺菌属%提取法%微生物学%图谱
誌賀菌屬%提取法%微生物學%圖譜
지하균속%제취법%미생물학%도보
Shigella%extraction%microbiology%pictorial works
目的：建立基质辅助激光解吸电离飞行时间质谱（matrix-assisted laser desorption/ionization-time of flight mass spectrometry, MALDI-TOF-MS）鉴定宋内志贺菌的质谱数据库，实现对临床分离宋内志贺菌的快速鉴定。方法建立经提取法处理后鉴定宋内志贺菌的质谱数据库。在分别使用直接涂抹法和提取法处理后对200株宋内志贺菌野生株进行鉴定验证；同时针对宋内志贺菌的不同菌相、不同激光强度和不同样本保存时间分别进行MALDI-TOF-MS鉴定。再选取100株大肠埃希菌，进行MALDI-TOF-MS鉴定，以确定新建数据库的特异性。结果宋内志贺菌经提取法处理后的鉴定符合率为100%，经直接涂抹法处理后的鉴定符合率为71.5%。宋内志贺菌不同菌相和不同激光强度的鉴定结果差异无统计学意义（P＞0.05）；而样本不同保存时间鉴定结果差异有统计学意义（P<0.05）。使用新数据库后，大肠埃希菌鉴定符合率为96.0%。结论经提取法处理后应用MALDI-TOF-MS鉴定宋内志贺菌建立的质谱数据库，能快速、准确鉴定宋内志贺菌到种水平，满足腹泻病原菌临床分离株的快速诊断。
目的：建立基質輔助激光解吸電離飛行時間質譜（matrix-assisted laser desorption/ionization-time of flight mass spectrometry, MALDI-TOF-MS）鑒定宋內誌賀菌的質譜數據庫，實現對臨床分離宋內誌賀菌的快速鑒定。方法建立經提取法處理後鑒定宋內誌賀菌的質譜數據庫。在分彆使用直接塗抹法和提取法處理後對200株宋內誌賀菌野生株進行鑒定驗證；同時針對宋內誌賀菌的不同菌相、不同激光彊度和不同樣本保存時間分彆進行MALDI-TOF-MS鑒定。再選取100株大腸埃希菌，進行MALDI-TOF-MS鑒定，以確定新建數據庫的特異性。結果宋內誌賀菌經提取法處理後的鑒定符閤率為100%，經直接塗抹法處理後的鑒定符閤率為71.5%。宋內誌賀菌不同菌相和不同激光彊度的鑒定結果差異無統計學意義（P＞0.05）；而樣本不同保存時間鑒定結果差異有統計學意義（P<0.05）。使用新數據庫後，大腸埃希菌鑒定符閤率為96.0%。結論經提取法處理後應用MALDI-TOF-MS鑒定宋內誌賀菌建立的質譜數據庫，能快速、準確鑒定宋內誌賀菌到種水平，滿足腹瀉病原菌臨床分離株的快速診斷。
목적：건립기질보조격광해흡전리비행시간질보（matrix-assisted laser desorption/ionization-time of flight mass spectrometry, MALDI-TOF-MS）감정송내지하균적질보수거고，실현대림상분리송내지하균적쾌속감정。방법건립경제취법처리후감정송내지하균적질보수거고。재분별사용직접도말법화제취법처리후대200주송내지하균야생주진행감정험증；동시침대송내지하균적불동균상、불동격광강도화불동양본보존시간분별진행MALDI-TOF-MS감정。재선취100주대장애희균，진행MALDI-TOF-MS감정，이학정신건수거고적특이성。결과송내지하균경제취법처리후적감정부합솔위100%，경직접도말법처리후적감정부합솔위71.5%。송내지하균불동균상화불동격광강도적감정결과차이무통계학의의（P＞0.05）；이양본불동보존시간감정결과차이유통계학의의（P<0.05）。사용신수거고후，대장애희균감정부합솔위96.0%。결론경제취법처리후응용MALDI-TOF-MS감정송내지하균건립적질보수거고，능쾌속、준학감정송내지하균도충수평，만족복사병원균림상분리주적쾌속진단。
objective To construct a database of Shigella sonnei isolates identified by matrix-assisted laser desorption/ioniza-tion-time of flight (MALDI-TOF-MS) in order to achieve rapid identification of clinically isolated Shigella sonnei. Methods A mass spectrometry database of Shigella sonnei isolates identified following extraction method was constructed. A total of 200 wild-type Shigella sonnei strains were identified following direct-smear method and extraction method, respectively. Meanwhile, the iden-tification by MALDI-TOF-MS was conducted for different phases of Shigella sonnei strains, at different laser intensity of the instru-ment and at different sample storage time. Then, 100 Escherichia Coli strains were identified to verify the specificity of the newly-constructed database. Results Concordance rates were 100% for the identification following extraction method, and 71.5% for the identification following direct-smear method. The results of the identification for different phases of Shigella sonnei strains and at dif-ferent laser intensity of the instrument were not significantly different (P>0.05) , and the results of the identification at different sam-ple storage time were significantly different (P<0.05). Identification concordance rate of Escherichia Coli were 96.0% based on the newly-constructed database. Conclusions Based on the database of Shigella sonnei isolates identified by MALDI-TOF-MS follow-ing extraction method, a rapid and correct identification of Shigella sonnei to the species level is obtained, and a rapid diagnosis of the clinical strains of the diarrheal pathogenic bacteria is realized.
