CAJ | 학술논문

为研究盐胁迫对棉花超微结构的影响，用透射电子显微镜对NaCl胁迫下的棉花叶片和根系进行了观察和分析。结果表明，未胁迫的棉花叶片及根系细胞中各细胞器结构完整，而0.3% NaCl胁迫3 d后，引起了6个棉花品种超微结构的变化，其中，中50、N52、N203和K-1叶绿体形状改变，膜变得模糊，基粒片层结构分散，其中有部分叶绿体受损极为严重，包括类囊体排列紊乱、扭曲、松散，部分类囊体膜解体、空泡化，甚至消失，嗜锇颗粒增多；而根细胞中线粒体数量增多，膜系统受到破坏，嵴减少甚至模糊，细胞核核膜、线粒体膜破裂，核内染色质高度凝缩，核发生降解，而中熟品种N177和N181受破坏较轻，有较为完整的线粒体和细胞壁。试验结果经综合分析显示，中熟棉花品种N177和N181具有与耐盐性相适应的解剖结构特点，其抗盐性较强。
위연구염협박대면화초미결구적영향，용투사전자현미경대NaCl협박하적면화협편화근계진행료관찰화분석。결과표명，미협박적면화협편급근계세포중각세포기결구완정，이0.3% NaCl협박3 d후，인기료6개면화품충초미결구적변화，기중，중50、N52、N203화K-1협록체형상개변，막변득모호，기립편층결구분산，기중유부분협록체수손겁위엄중，포괄류낭체배렬문란、뉴곡、송산，부분류낭체막해체、공포화，심지소실，기철과립증다；이근세포중선립체수량증다，막계통수도파배，척감소심지모호，세포핵핵막、선립체막파렬，핵내염색질고도응축，핵발생강해，이중숙품충N177화N181수파배교경，유교위완정적선립체화세포벽。시험결과경종합분석현시，중숙면화품충N177화N181구유여내염성상괄응적해부결구특점，기항염성교강。
The ultrastructures of leaf and root in six cotton varieties under NaCl stress were studied using trans-mission electron microscope. The results showed that various organelles of mesophyll and root maintained morpholog-ical integrity under normal condition, while their ultrastructure changed under 0. 3% NaCl stress. In mesophyll cells,the chloroplasts of Zhong 50,N52,N203 and K-1 began deformed,with ambiguity in membrane and disorgani-zation in grana lamell. One part of chloroplasts was seriously destroyed. The arrangement of thylakoid was disorder-ed,loosened and twisted. Some of the thylakoid membrane disintegrated and cavitated gradually,even disappeared. The hungry particles increased significantly. In root cells,the numbers of mitochondrion increased and the membrane systems destroyed. The cristaes of mitochondrion were reduced and even vague. The cytoplasm segregated from the cell wall,and the lamellae of nucleolus and mitochondrion suffered disruption. Meanwhile,the chromatin was poly-merized and the cell nuclear was degraded. But the mid-maturing varieties N177 and N181 have been undamaged. The mitochondrion and the cell walls had been maintained morphological structure integrity completely. Comparative analysis indicated that the mid-maturing varieties N177 and N181 had anatomic adaptation to the salt stress. The a-bove results showed that the salt resistance of N177 and N181 was stronger than the other four varieties.