CAJ | 학술논문

通过对荧光定量PCR反应条件的优化,建立了一种检测PCV2的SYBR Green荧光定量PCR方法。试验结果表明,该方法特异性强,与猪1型圆环病毒、猪流感病毒、猪繁殖与呼吸综合征病毒、猪伪狂犬病毒等均无交叉反应。该方法最低可检测到10拷贝/μL的DNA,比PCR检测方法敏感性高1000倍；其标准曲线线性范围是6.84×102~6.84×107拷贝,且具有良好的重复性。
통과대형광정량PCR반응조건적우화,건립료일충검측PCV2적SYBR Green형광정량PCR방법。시험결과표명,해방법특이성강,여저1형원배병독、저류감병독、저번식여호흡종합정병독、저위광견병독등균무교차반응。해방법최저가검측도10고패/μL적DNA,비PCR검측방법민감성고1000배；기표준곡선선성범위시6.84×102~6.84×107고패,차구유량호적중복성。
By quantitative PCR optimization of reaction conditions,a real-time detection of PCV2 SYBR Green quantitative PCR method was established. The results indicated that the method was specificity,the porcine circovir-us type 1(PCV1),swine influenza virus(SIV),porcine reproductive and respiratory syndrome virus(PRRSV),pig pseudorabies virus( PRV) and other common diseases of the original pig detection results were negative. The detec-tion limit of the assay was 10 copies/μL of plasmid DNA,1 000 times higher than that of the routine PCR. The standard curve displayed a linear range from 6. 84 × 102 to 6. 84 × 107 copies and a good reproducibility.