CAJ | 학술논문

利用RT-PCR方法从猪沙波病毒CH430株中克隆出非结构蛋白p70基因并测序,结果显示,p70基因全长1995 nt,编码665个氨基酸,经同源性比对及遗传进化树分析表明,CH430株为基因Ⅲ型沙波病毒毒株。生物信息学分析显示该蛋白理论等电点(pI)为5.96,理论分子质量为72876.6 u；其序列上共发现25个磷酸化位点,分别为Ser (6)、Thr(13)、Tyr(6),而蛋白的磷酸化与信号传导有关,预测该蛋白为一重要的信号传导分子；无信号肽和跨膜区；该蛋白含有胰蛋白酶样丝氨酸蛋白酶和RdRp功能域；胰蛋白酶样丝氨酸蛋白酶功能域核心结构为1个由7个β折叠形成柱状结构和1个由1个α螺旋和7个β折叠扭曲平行形成1个不完全的桶状结构共同组成,RdRp功能域核心结构为1个具有3个子结构域空间结构,该结构与右手掌在形态上非常相似,而3个子结构域也被认为是手指、手掌和拇指。
이용RT-PCR방법종저사파병독CH430주중극륭출비결구단백p70기인병측서,결과현시,p70기인전장1995 nt,편마665개안기산,경동원성비대급유전진화수분석표명,CH430주위기인Ⅲ형사파병독독주。생물신식학분석현시해단백이론등전점(pI)위5.96,이론분자질량위72876.6 u；기서렬상공발현25개린산화위점,분별위Ser (6)、Thr(13)、Tyr(6),이단백적린산화여신호전도유관,예측해단백위일중요적신호전도분자；무신호태화과막구；해단백함유이단백매양사안산단백매화RdRp공능역；이단백매양사안산단백매공능역핵심결구위1개유7개β절첩형성주상결구화1개유1개α라선화7개β절첩뉴곡평행형성1개불완전적통상결구공동조성,RdRp공능역핵심결구위1개구유3개자결구역공간결구,해결구여우수장재형태상비상상사,이3개자결구역야피인위시수지、수장화무지。
The non-structural protein p70 gene of PoSaV CH430 strain was cloned by RT-PCR and then se-quenced in our study. The results showed that the full length of p70 gene was 1 995 nt encoding a protein of 665 aa. Homology alignment and evolutionary tree showed that PoSaV CH430 strain belong to GⅢ. The bioinformatics anal-ysis demonstrated that the isoelectric point and molecular weight of non-structural protein p70 were 5 . 96 and 72 876. 6 u. The protein had no signal peptide and transmenbrane domain. There were 25 phosphorylation sites inclu-ding 6 Sers,13 Thrs and 6 Tyrs. Protein phosphorylation was concerned with signal transduction,so this protein may be a signaling molecule. There were Trypsin-like serine proteases and RdRp functional domain. Main structure of trypsin-like serine proteases functional domain was a column structure and an incompleteβ-barrel. Main structure of RdRp functional domain was a fold whose organisation has been likened to the shape of a right hand with three sub-domains termed fingers,palm and thumb.