CAJ | 학술논문

为了探讨乙烯利调控玉米生长的分子机理，以郑单958为材料，拔节期叶面喷施225 mL/hm2浓度的乙烯利，同体积清水为对照。在不同时间取茎端分生组织样品，采用cDNA-AFLP( RT-PCR)进行基因差异表达分析。结果表明，利用70条引物共扩增1635条片段，其中上调表达的有600条，占总条带的36.7%；下调表达的有564条，占总条带的34.5%；无差异表达的有471条，占总条带数的28.8%。选择重复扩增稳定的30条差异片断进行回收测序，经过BlastX比对分析这些差异表达基因片段，按功能可分为信号转导相关基因(6.7%)、抗性相关基因(16.7%)、能量与代谢相关基因(20.0%)、转录因子相关基因(10.0%)、未知功能蛋白(13.3%)和未知基因(33.3%)6大类。乙烯利通过调控谷胱甘肽S-转移酶、天冬氨酸蛋白激酶和生长素诱导蛋白等基因的表达来调控玉米的生长。
위료탐토을희리조공옥미생장적분자궤리，이정단958위재료，발절기협면분시225 mL/hm2농도적을희리，동체적청수위대조。재불동시간취경단분생조직양품，채용cDNA-AFLP( RT-PCR)진행기인차이표체분석。결과표명，이용70조인물공확증1635조편단，기중상조표체적유600조，점총조대적36.7%；하조표체적유564조，점총조대적34.5%；무차이표체적유471조，점총조대수적28.8%。선택중복확증은정적30조차이편단진행회수측서，경과BlastX비대분석저사차이표체기인편단，안공능가분위신호전도상관기인(6.7%)、항성상관기인(16.7%)、능량여대사상관기인(20.0%)、전록인자상관기인(10.0%)、미지공능단백(13.3%)화미지기인(33.3%)6대류。을희리통과조공곡광감태S-전이매、천동안산단백격매화생장소유도단백등기인적표체래조공옥미적생장。
To investigate the molecular mechanisms of ethephon-induced stalk internodes shorten in maize,u-sing maize variety Zhengdan 958,the plant leaves were treated with spraying ethephon(ETH) at 225 mL/ha at early elongation stage,and spraying water as a control. Samples of young stalks were taken and the differentially expressed genes were analyzed with cDNA-AFLP technique. The results showed that 1 635 fragments of expressed genes were obtained with 70 pairs of primers. The expressions of 600 genes were up-regulated(36. 7% of the total bands),564 genes down-regulated(34. 5% of the total bands) and the same expressions of 471 genes(28. 8% of the total bands) by ethephon induction. Through the BlastX analysis of 30 different gene fragments of TDFs can be divided into six categories by functional analysis,including signal transduction-related genes(accounting for 6. 7%),resist-ance-related genes(accounting for 16. 7%),energy and metabolism-related genes(accounting for 20. 0%),tran-scription factor-related genes(accounting for 10%),unknown functional proteins(accounting for 13. 3%) and un-known genes(accounting for 33. 3%). Ethephon influence the growth of maize through regulating the glutathione S-transferase,aspartic acid protein kinase and auxin-induced protein gene expressions.