国际妇产科学杂志
國際婦產科學雜誌
국제부산과학잡지
JOURNAL OF INTERNATIONAL OBSTETRICS AND GYNECOLOGY
2014年
3期
321-323
,共3页
宫颈肿瘤%宫颈上皮内瘤样病变%肿瘤坏死因子α%逆转录聚合酶链反应%乳头状瘤病毒科
宮頸腫瘤%宮頸上皮內瘤樣病變%腫瘤壞死因子α%逆轉錄聚閤酶鏈反應%乳頭狀瘤病毒科
궁경종류%궁경상피내류양병변%종류배사인자α%역전록취합매련반응%유두상류병독과
Uterine cervical neoplasms%Cervical intraepithelial neoplasia%Tumor necrosis factor-alpha%Reverse transcriptase polymerase chain reaction%Papillomaviridae
目的：检测肿瘤坏死因子α（TNF-α）及高危型人乳头瘤病毒（HR-HPV）E2、E6、E7基因在不同宫颈病变组织中的表达并分析其相关性。方法：根据不同病理结果把标本分为HPV阴性正常组，HPV16阳性正常组，HPV16阳性宫颈内瘤变（CIN）Ⅰ～Ⅱ组，HPV16阳性CINⅢ组以及HPV16阳性宫颈腺癌/鳞状细胞癌（CIS/SCC）组。通过反转录聚合酶链反应（RT-PCR）方法对各组标本进行TNF-α、E2、E6及E7蛋白的检测，并对检测结果进行统计学分析。结果：HPV E6、E7基因在不同宫颈病变中的表达逐渐升高，且E7的逐级升高趋势更显著，E2升高至CINⅡ后出现下降，而TNF-α在不同宫颈病变中的表达虽呈升高趋势，但在宫颈浸润癌中与CINⅢ组中表达差异无统计学意义。结论：用E2/E7的比值来预测或诊断高度宫颈病变程度可能更准确。炎症因子TNF-α的升高可能加重了HPV E2基因的破坏，使得HR-HPV E6、E7癌基因过度表达，从而促进宫颈癌发生。
目的：檢測腫瘤壞死因子α（TNF-α）及高危型人乳頭瘤病毒（HR-HPV）E2、E6、E7基因在不同宮頸病變組織中的錶達併分析其相關性。方法：根據不同病理結果把標本分為HPV陰性正常組，HPV16暘性正常組，HPV16暘性宮頸內瘤變（CIN）Ⅰ～Ⅱ組，HPV16暘性CINⅢ組以及HPV16暘性宮頸腺癌/鱗狀細胞癌（CIS/SCC）組。通過反轉錄聚閤酶鏈反應（RT-PCR）方法對各組標本進行TNF-α、E2、E6及E7蛋白的檢測，併對檢測結果進行統計學分析。結果：HPV E6、E7基因在不同宮頸病變中的錶達逐漸升高，且E7的逐級升高趨勢更顯著，E2升高至CINⅡ後齣現下降，而TNF-α在不同宮頸病變中的錶達雖呈升高趨勢，但在宮頸浸潤癌中與CINⅢ組中錶達差異無統計學意義。結論：用E2/E7的比值來預測或診斷高度宮頸病變程度可能更準確。炎癥因子TNF-α的升高可能加重瞭HPV E2基因的破壞，使得HR-HPV E6、E7癌基因過度錶達，從而促進宮頸癌髮生。
목적：검측종류배사인자α（TNF-α）급고위형인유두류병독（HR-HPV）E2、E6、E7기인재불동궁경병변조직중적표체병분석기상관성。방법：근거불동병리결과파표본분위HPV음성정상조，HPV16양성정상조，HPV16양성궁경내류변（CIN）Ⅰ～Ⅱ조，HPV16양성CINⅢ조이급HPV16양성궁경선암/린상세포암（CIS/SCC）조。통과반전록취합매련반응（RT-PCR）방법대각조표본진행TNF-α、E2、E6급E7단백적검측，병대검측결과진행통계학분석。결과：HPV E6、E7기인재불동궁경병변중적표체축점승고，차E7적축급승고추세경현저，E2승고지CINⅡ후출현하강，이TNF-α재불동궁경병변중적표체수정승고추세，단재궁경침윤암중여CINⅢ조중표체차이무통계학의의。결론：용E2/E7적비치래예측혹진단고도궁경병변정도가능경준학。염증인자TNF-α적승고가능가중료HPV E2기인적파배，사득HR-HPV E6、E7암기인과도표체，종이촉진궁경암발생。
Objective:To detect TNF-α,E2,E6 and E7 in different cervical lesion and analyse the relationship between them. Methods:According to different pathology result, specimens were divided into normal group of HPV negative,HPV16 positive normal group,HPV16 positive CINⅠ-Ⅱ,HPV16 positive CINⅢ,HPV16 positive SCC group. TNF-α,E2,E6 and E7 proteins of each group specimens were detected by using RT-PCR ,and the result of the test were analyzed statistically. Results:HPV E6 and E7 gene expression is positively correlated with the malignant degree of cervical cells ,and the E7 gradually rising trend is more significant. The E2 fall after rise to CINⅡ,and of TNF-αbasic is the rising trend in different cervical lesions ,but the expression has no obvious difference between CINⅢgroup and cervical invasive cancer. Conclusions:With the ratio of E2/E7 to predict or diagnose the degree of high cervical lesions might be more accurate. Elevated inflammatory cytokines of TNF-αmay increase the destruction of E2, which lead to excessive expression of oncogene E6 and E7 ,thus promote the occurrence of cervical cancer.
