CAJ | 학술논문

目的：研究左炔诺孕酮纳米载药聚乳酸（PLA）-聚苯乙烯（PS）复合材料对人精子活力及超微结构影响。方法正常生育男性精液，经密度梯度离心处理后分为空膜组、载药膜组和正常对照组，分别与PLA-PS复合生物膜（空膜）、左炔诺孕酮纳米载药PLA-PS复合生物膜（载药膜）共培养24h，以未加膜培养的精子作为对照，计算机辅助精液分析（CASA）检测精子活力，透射电镜下观察各组精子超微结构的变化。结果（1）共培养24h后各组间精子活力差异无明显统计学意义（P＞0.05）；（2）空膜组精子的超微结构与对照组无明显差别；（3）载药膜组精子的顶体波浪状改变，顶体缺损或消失，质膜溶解，线粒体空泡样变，凋亡精子及崩解精子与空膜组及对照组比较明显增加。结论 PLA-PS复合材料本身对人精子活力及超微结构无明显影响，加入左炔诺孕酮纳米载药的PLA-PS复合材料可使精子超微结构改变，促进精子凋亡，为左炔诺孕酮纳米载药PLA-PS复合材料作为一种安全有效的新型生物可降解纳米载药宫内节育系统提供了进一步的实验依据。
목적：연구좌결낙잉동납미재약취유산（PLA）-취분을희（PS）복합재료대인정자활력급초미결구영향。방법정상생육남성정액，경밀도제도리심처리후분위공막조、재약막조화정상대조조，분별여PLA-PS복합생물막（공막）、좌결낙잉동납미재약PLA-PS복합생물막（재약막）공배양24h，이미가막배양적정자작위대조，계산궤보조정액분석（CASA）검측정자활력，투사전경하관찰각조정자초미결구적변화。결과（1）공배양24h후각조간정자활력차이무명현통계학의의（P＞0.05）；（2）공막조정자적초미결구여대조조무명현차별；（3）재약막조정자적정체파랑상개변，정체결손혹소실，질막용해，선립체공포양변，조망정자급붕해정자여공막조급대조조비교명현증가。결론 PLA-PS복합재료본신대인정자활력급초미결구무명현영향，가입좌결낙잉동납미재약적PLA-PS복합재료가사정자초미결구개변，촉진정자조망，위좌결낙잉동납미재약PLA-PS복합재료작위일충안전유효적신형생물가강해납미재약궁내절육계통제공료진일보적실험의거。
Objective To investigate the effects of levonorgestrel-loaded nanoparticle composites on human sperm motility and ultrastructure. Methods Semen samples from normal reproductive males were collected anddivided into empty biofilm (polylactic acid-polystyrene composite biofilm) group, drug-loaded biofilm(levonorgestrel-loaded nanoparticle polylactic acid-polystyrene composite biofilm) group and normal control group. After co-culture with empty biofilm or drug-loaded biofilm for 24 hours, sperm motilityand sperm ultrastructure were detected by CASA and transmission electron microscope respectively. Results (1) No significant differences were observed in sperm motility among groups. (2) Compared with that of the control group, no obvious difference in sperm ultrastructure was found in empty biofilm group. (3) Remarkable differences were observed in drug-loaded biofilmgroup such as wavy change, defect or disappearance of acrosome, plasma membrane dissolved, vacuolar degeneration of mitochondria, as well as a significant increase in sperm apoptosis and disintegration of sperm. Conclusion Polylactic acid - polystyrene composites itself has no effects on human sperm motility and ultrastructure, levonorgestrel-loaded nanoparticle polylactic acid-polystyrene composites has obvious effect on human sperm acrosome, plasma membrane, mitochondria and promotes sperm apoptosis, which provides the theoretical basis for further study of biodegradable sustained-released nanoparticle drug-loaded intrauterine contraceptive system.