CAJ | 학술논문

目的：观察温胆汤对荷瘤小鼠的胃肠道5-羟色胺和嗜铬细胞分泌的影响。方法皮下注射 Colon-26腺瘤建立小鼠荷瘤模型。将32只小鼠随机分为温胆汤组和荷瘤对照组。在第7、14天用高效液相色谱法测定小肠中5-羟色胺的含量，用免疫组化法检测嗜铬细胞的密度。并观察每组小鼠的摄食量。结果正常小鼠第1周和第2周小肠的5-羟色胺含量分别为（1．57±0．09）μg·g-1和（1．54±0．11）μg·g-1，而接种肿瘤细胞后的小鼠分别为（2．93±0．17）μg·g-1和（3．99±0．32）μg·g-1，显著高于正常小鼠。而温胆汤组则分别降到（2．03±0．17）μg· g-1和（3．05±0．17）μg·g-1，显著低于荷瘤对照组，2组比较差异有统计学意义（P＜0．01）。免疫组化显示小肠中嗜铬细胞在肿瘤细胞移植后的第1周和第2周显著增加，而服用温胆汤的小鼠小肠嗜铬细胞数量明显降低，2组比较差异有统计学意义（P＜0．01）。在第14天，荷瘤组小鼠和温胆汤组小鼠的平均食量分别为（0．70±0．12）g和（1．12±0．04）g，2组比较差异有统计学意义（P＜0．01）。结论温胆汤可以通过减少肿瘤患者肠道嗜铬细胞数量和5-羟色胺含量而减轻肿瘤相关胃肠道反应。
목적：관찰온담탕대하류소서적위장도5-간색알화기락세포분비적영향。방법피하주사 Colon-26선류건립소서하류모형。장32지소서수궤분위온담탕조화하류대조조。재제7、14천용고효액상색보법측정소장중5-간색알적함량，용면역조화법검측기락세포적밀도。병관찰매조소서적섭식량。결과정상소서제1주화제2주소장적5-간색알함량분별위（1．57±0．09）μg·g-1화（1．54±0．11）μg·g-1，이접충종류세포후적소서분별위（2．93±0．17）μg·g-1화（3．99±0．32）μg·g-1，현저고우정상소서。이온담탕조칙분별강도（2．03±0．17）μg· g-1화（3．05±0．17）μg·g-1，현저저우하류대조조，2조비교차이유통계학의의（P＜0．01）。면역조화현시소장중기락세포재종류세포이식후적제1주화제2주현저증가，이복용온담탕적소서소장기락세포수량명현강저，2조비교차이유통계학의의（P＜0．01）。재제14천，하류조소서화온담탕조소서적평균식량분별위（0．70±0．12）g화（1．12±0．04）g，2조비교차이유통계학의의（P＜0．01）。결론온담탕가이통과감소종류환자장도기락세포수량화5-간색알함량이감경종류상관위장도반응。
Objective To observe the effects of Wendan decoction on serotonin production and chromaffin cell secretion in the gastrointestinal tract in tumor-bearing mice.Methods Colon-26 carcinoma cells were inj ected subcutaneously into mice to establish the tumor-bearing model. Thirty-two tumor-bearing mice were randomly divided into Wendan decoction treatment group and tumor-bearing group.The content of serotonin in small intestine was determined by HPLC and the number of enterochromaffin cells was measured by immunochemistry at 7 and 14 days af-ter inoculation of tumor cells.In addition,food intake was measured.Results At 7 and 14 days after inoculation of tumor cells,the contents of serotonin in small intestine were,respectively,(1 .5 7 ± 0.09)and (1.54±0.11)μg·g-1 in normal mice,(2.93±0.17)and (3.99±0.32)μg·g-1 in tumor-bearing group,and(2.03±0.17)and (3.05±0.17)μg·g-1 in Wendan decoction treat-ment group.The differences in serotonin content were significant among the three groups (P<0.01).Compared normal controls,the number of enterochromaffin cells significantly increased in tumor-bearing group at 7 and 14 days after inoculation of tumor cells(P<0.01).However,Wen-dan decoction treatment significantly decreased the number of enterochromaffin cells compared with tumor-bearing group (P<0.01 ).Moreover,Wendan decoction treatment significantly de-creased the food intake compared with tumor-bearing group at 14 days after inoculation of tumor cells(P<0.01).Conclusion Wendan decoction treatment can alleviate tumor-associated gastroin-testinal tract reaction through reducing the number of enterochromaffin cells and the content of serotonin.