CAJ | 학술논문

目的：探讨脐带（UC）间充质干细胞（MSC）生物学的特性。方法行剖宫产术时采集胎儿 UC，采用1 g· L-1Ⅱ型胶原酶消化、分离 UC-MSC。将第3代 UC-MSC随机分为3组：正常对照组、诱导向脂肪细胞分化组和诱导向软骨细胞分化组，分别在光学显微镜下观察 UC-MSC形态，流式细胞仪检测 UC-MSC表面标志，体外诱导UC-MSC分化为软骨及脂肪细胞。结果第3代 UC-MSC 经流式细胞仪测定证明，其高表达 CD29、CD105、CD166和CD90，不表达CD34、CD45。正常对照组的 UC-MSC细胞呈长梭形，排列有明显方向性；诱导向脂肪细胞分化组的 UC-MSC细胞呈圆形或近似圆形；诱导向软骨细胞分化组的 UC-MSC细胞呈梭形，也有呈圆形或近似圆形。油红 O染色证明 UC-MSC 经诱导分化成脂肪细胞，阿新兰染色 UC-MSC 经诱导分化成软骨细胞。结论UC-MSC 具有多能性，其可作为一种新的 MSC来源，并可用于临床干细胞移植。
목적：탐토제대（UC）간충질간세포（MSC）생물학적특성。방법행부궁산술시채집태인 UC，채용1 g· L-1Ⅱ형효원매소화、분리 UC-MSC。장제3대 UC-MSC수궤분위3조：정상대조조、유도향지방세포분화조화유도향연골세포분화조，분별재광학현미경하관찰 UC-MSC형태，류식세포의검측 UC-MSC표면표지，체외유도UC-MSC분화위연골급지방세포。결과제3대 UC-MSC 경류식세포의측정증명，기고표체 CD29、CD105、CD166화CD90，불표체CD34、CD45。정상대조조적 UC-MSC세포정장사형，배렬유명현방향성；유도향지방세포분화조적 UC-MSC세포정원형혹근사원형；유도향연골세포분화조적 UC-MSC세포정사형，야유정원형혹근사원형。유홍 O염색증명 UC-MSC 경유도분화성지방세포，아신란염색 UC-MSC 경유도분화성연골세포。결론UC-MSC 구유다능성，기가작위일충신적 MSC래원，병가용우림상간세포이식。
Objective To study the biological characteristics of mesenchymal stem cells derived from umbilical cord(UC-MSCs).Methods The umbilical cords were obtained from pregnancies delivered by caesarean section.UC-MSCs were isolated by typeⅡ collagenase digestion.The UC-MSCs at the third generation were randomly divided into three groups:normal control group,dif-ferentiation into adipocyte group and differentiation into chondrocyte group.The morphology of UC-MSCs was observed under an optical microscope.The surface markers of UC-MSCs were de-tected by flow cytometry.In addition,the differentiation of UC-MSCs into adipocytes and chon-drocytes were observed.Results Flow cytometry showed that UC-MSCs highly expressed CD29, CD105,CD166 and CD90 but did not express CD34 and CD45.UC-MSCs showed spindle shape and arranged with obvious directivity.Cells differentiated toward adipocytes showed round or ap-proximately round shape.Cells differentiated toward chondrocytes showed spindle,round or ap-proximately round shape.Moreover,cells differentiated into adipocytes and chondrocytes were proved by oil red O staining and alcian blue staining,respectively.Conclusion UC-MSCs are plu-ripotent cells that may be used as a new source for stem cell transplantation.