CAJ | 학술논문

目的：探讨5-异丁基-3-（2-氯）苄氧基乙内酰脲（YL3）对白血病细胞 K562细胞生长与凋亡的作用。方法将K562细胞置于含10％FBS的 RPMI-1640培养液中培养。实验分4个组：空白对照组、阴性对照组、阳性对照组（HU组）、受试药物组（YL3组）；除空白对照组外，其余3组均接种K562细胞；HU组、YL3组分5个亚组，分别加入10-3～10-7 mol·L-15个梯度浓度的 HU或 YL3。采用 MTT法检测 YL3对 K562细胞增殖的影响；AV/PI双染流式细胞术检测 YL3对 K562细胞凋亡的影响；瑞氏染色法观察细胞形态的变化。结果 YL3在10-3～10-6 mol·L-1浓度范围内显著抑制 K562细胞增殖（P＜0．01或 P＜0．05），其半数细胞抑制浓度（IC50）值为88．5μmol·L-1；YL3有促进K562细胞凋亡的作用，其高剂量的凋亡率显著高于同剂量的 HU组（P＜0．01），且呈量效关系（P＜0．05）。结论5-异丁基-3-（2-氯）苄氧基乙内酰脲具有抑制 K562细胞增殖，诱导其凋亡的作用。
목적：탐토5-이정기-3-（2-록）변양기을내선뇨（YL3）대백혈병세포 K562세포생장여조망적작용。방법장K562세포치우함10％FBS적 RPMI-1640배양액중배양。실험분4개조：공백대조조、음성대조조、양성대조조（HU조）、수시약물조（YL3조）；제공백대조조외，기여3조균접충K562세포；HU조、YL3조분5개아조，분별가입10-3～10-7 mol·L-15개제도농도적 HU혹 YL3。채용 MTT법검측 YL3대 K562세포증식적영향；AV/PI쌍염류식세포술검측 YL3대 K562세포조망적영향；서씨염색법관찰세포형태적변화。결과 YL3재10-3～10-6 mol·L-1농도범위내현저억제 K562세포증식（P＜0．01혹 P＜0．05），기반수세포억제농도（IC50）치위88．5μmol·L-1；YL3유촉진K562세포조망적작용，기고제량적조망솔현저고우동제량적 HU조（P＜0．01），차정량효관계（P＜0．05）。결론5-이정기-3-（2-록）변양기을내선뇨구유억제 K562세포증식，유도기조망적작용。
Objective To explore the effect of 3-(2-chlorobenzyloxy )-5-isobutylhydantoin (YL3)on the growth and apoptosis of leukemia K562 cells.Methods Leukemia K562 cells were cultured in RPMI-1640 supplemented with 10% fetal bovine serum.Cells were divided into four groups:blank control group,negative control group,positive control group(HU group)and YL3 group.Furthermore,cells in HU group and YL3 group were treated with HU and YL3 at concen-trations of 10-3-10-7 mol·L-1,respectively.The proliferation and apoptosis were detected by MTT assay and A V/PI flow cytometry,respectively.Changes in cellular morphology were ob-served by Wight-Giemsa staining.Results YL3 treatment(10-3-10-6 mol·L-1 )significantly in-hibited the growth of leukemia K562 cells(P<0.01 or P<0.05).The half-maximal inhibitory concentration(IC50)was 88.5μmol·L-1.In addition,YL3 promoted the apoptosis of leukemia K562 cells in a dose-dependent manner(P<0.05),and the apoptosis rate in high-dose YL3 group was significantly higher than that in high-dose HU group(P<0.01).Conclusion YL3 treatment can inhibit the growth and induce the apoptosis in leukemia K562 cells.