CAJ | 학술논문

【目的】探讨肿瘤坏死因子α（TNF-α）基因-857C/T 和-1031C/T 两个位点单核苷酸多态性（SNP）与妊娠期糖尿病之间的关系。【方法】选取2012年3月至2013年2月北京航天总医院50例正常孕妇（对照组）及50例妊娠期糖尿病（GDM组）孕妇，抽取外周静脉血，提取DNA，采用PCR限制性酶切片段长度分析-857C/T和-1031C/T，测序验证。应用χ2检验比较基因型分布是否符合 Hardy-Weinberg平衡。采用t检验和χ2检验等统计学处理，判断结果是否具统计学意义。【结果】GDM组 TNF-α基因-857C/T位点的基因型分布及等位基因频率和正常对照组比较差异具有显著性（P ＜0．05）。在 GDM组中 CT基因型所占比例及T等位基因频率远远高于正常孕妇组（P ＜0．05）；GDM组-1031 C/T位点中基因型分布及等位基因频率和对照组相比差异均无显著性（P＞0．05）。【结论】TNF-α基因-857C/T等位基因多态性与 GDM的发病有关，具有T位点者罹患妊娠期糖尿病；-1031C/T等位基因位点与妊娠期糖尿病无关。
【목적】탐토종류배사인자α（TNF-α）기인-857C/T 화-1031C/T 량개위점단핵감산다태성（SNP）여임신기당뇨병지간적관계。【방법】선취2012년3월지2013년2월북경항천총의원50례정상잉부（대조조）급50례임신기당뇨병（GDM조）잉부，추취외주정맥혈，제취DNA，채용PCR한제성매절편단장도분석-857C/T화-1031C/T，측서험증。응용χ2검험비교기인형분포시부부합 Hardy-Weinberg평형。채용t검험화χ2검험등통계학처리，판단결과시부구통계학의의。【결과】GDM조 TNF-α기인-857C/T위점적기인형분포급등위기인빈솔화정상대조조비교차이구유현저성（P ＜0．05）。재 GDM조중 CT기인형소점비례급T등위기인빈솔원원고우정상잉부조（P ＜0．05）；GDM조-1031 C/T위점중기인형분포급등위기인빈솔화대조조상비차이균무현저성（P＞0．05）。【결론】TNF-α기인-857C/T등위기인다태성여 GDM적발병유관，구유T위점자리환임신기당뇨병；-1031C/T등위기인위점여임신기당뇨병무관。
[Objective]To explore the association between singe nucleotide polymorphism(SNP)of two lo-ci -857C/T and -1031C/T of tumor necrosis factor-α(TNF-α)gene and gestational diabetes mellitus (GDM).[Methods]Totally 50 normal pregnant women(control group)and 50 pregnant women with GDM (group GDM)in Beijing aerospace general hospital from March 2012 to Feb 2013 were selected.Peripheral ve-nous blood was collected to extract DNA.Polymerase chain reaction(PCR)restriction enzyme fragment length was used to analyze -857C/T and -1031C/T.The sequencing was performed for the confirmation.Chi-square test was applied to confirm the Hardy-Weinberg equilibrium in genotype distribution.Statistical meth-ods involving t test and chi-square test were conducted to evaluate the statistical significance in all data.[Re-sults]There was significant difference in genotype distribution and allele frequency of-857C/T of TNF-αgene between GDM group and control group(P<0.05).The proportion of CT genotype and the frequency of allele genotype T in GDM group was significant higher than that in normal pregnant group(P<0.05).However, there was no significant difference in genotype distribution and allele frequency of -1031 C/T locus between GDM group and control group(P >0.05).[Conclusion]The -857C/T allele gene polymorphism of TNF-αgene is associated with the pathogenesis of GDM.Pregnant women with T locus are susceptible to have GDM. But -1031C/T polymorphism is not related with GDM.