CAJ | 학술논문

【目的】探讨RNA干扰抑制内膜癌RL95-2细胞XIAP基因表达及对细胞凋亡的影响。【方法】设计并合成XIAP基因特异性siRNA，转染人内膜癌RL95-2细胞，Real time RT-PCR检测转染后XIAP mRNA的变化，Western Blot检测 XIAP蛋白的变化，MTT 及流式细胞仪法检测细胞增值和凋亡的变化。【结果】XIAP siRNA转染后，特异性转染组mRNA转录量的相对倍数值为0．04±0．06，相对蛋白表达量分别为0．590±0．178，较各对照组明显减少（P＜0．05）；特异性转染组细胞生长抑制率为（47．86±4．46）％，较对照组明显增高（P＜0．05）。【结论】体外实验表明，合成的 siRNA能在mRNA水平及蛋白水平有效抑制人内膜癌RL95-2细胞内 XIAP基因的转录及表达，进而显著的促进内膜癌细胞凋亡，其促凋亡机制仍有待进一步研究。
【목적】탐토RNA간우억제내막암RL95-2세포XIAP기인표체급대세포조망적영향。【방법】설계병합성XIAP기인특이성siRNA，전염인내막암RL95-2세포，Real time RT-PCR검측전염후XIAP mRNA적변화，Western Blot검측 XIAP단백적변화，MTT 급류식세포의법검측세포증치화조망적변화。【결과】XIAP siRNA전염후，특이성전염조mRNA전록량적상대배수치위0．04±0．06，상대단백표체량분별위0．590±0．178，교각대조조명현감소（P＜0．05）；특이성전염조세포생장억제솔위（47．86±4．46）％，교대조조명현증고（P＜0．05）。【결론】체외실험표명，합성적 siRNA능재mRNA수평급단백수평유효억제인내막암RL95-2세포내 XIAP기인적전록급표체，진이현저적촉진내막암세포조망，기촉조망궤제잉유대진일보연구。
[Obj ective]To explore the effect of RNA interference(RNAi)targeting XIAP gene on cell ap-optosis of human endometrial carcinoma.[Methods]Specific small interference RNA(siRNA)of XIAP was de-signed and composed.Endometrial cancer cell line RL95-2 was transfected.The changes of mRNA and protein of XIAP after transfection were detected by real time RT-PCR and Western Blotting,respectively.MTT and flow cytometry method were used to detect cell proliferation and apoptosis.[Results]After transfection of XI-AP siRNA,the relative fold value of mRNA transcription volume and the relative protein expression volume in specific transfection group were (0.04±0.06)and (0.590±0.178)respectively,which were obviously lower than those in the control group(P<0.05).The cell growth inhibition rate in specific transduction group was (47.86±4.46)%,which was obviously higher than that in control group(P<0.05).[Conclusion]The ex-periment in vitro indicates that the composed siRNA can effectively inhibit the transcription and expression of XIAP in human endometrial cancer cell line RL95-2,and further promote cell apoptosis of endometrial carcino-ma significantly.The mechanism of promoting cell apoptosis still needs further investigation.