CAJ | 학술논문

目的：建立测定人血浆中硝苯地平的高效液相色谱-质谱联用（ HPLC-MS/MS）方法。方法血浆样品经乙腈沉淀蛋白提取分离，色谱柱为Utimat AQ-C18，流动相为乙腈-20 mmol · L-1醋酸铵（58∶42），流速为0.30 mL· min-1，柱温：40℃，电喷雾负离子源（ ESI -），用多反应离子检测（ MRM）。结果硝苯地平线性范围为0.03～80.00 ng · mL-1，定量下限为0.03 ng · mL-1，提取回收率在94.7％～102.3％，日内、日间RSD均小于9.8％。结论本方法专属性强，灵敏度高，操作简便、快速、准确，适用于硝苯地平缓释片药代动力学研究。
목적：건립측정인혈장중초분지평적고효액상색보-질보련용（ HPLC-MS/MS）방법。방법혈장양품경을정침정단백제취분리，색보주위Utimat AQ-C18，류동상위을정-20 mmol · L-1작산안（58∶42），류속위0.30 mL· min-1，주온：40℃，전분무부리자원（ ESI -），용다반응리자검측（ MRM）。결과초분지평선성범위위0.03～80.00 ng · mL-1，정량하한위0.03 ng · mL-1，제취회수솔재94.7％～102.3％，일내、일간RSD균소우9.8％。결론본방법전속성강，령민도고，조작간편、쾌속、준학，괄용우초분지평완석편약대동역학연구。
Objective To establish a HPLC-MS/MS method for deter-mination of nifedipine in human plasma.Methods After protein precip-itation, the plasma was separated on an Utimat AQ -C18 column, with a mobile phase of acetonitrile -20 mmol · L-1 ammonium acetate ( 58∶42 ) .The flow rate was 0.30 mL · min-1 and the column temperature 40 ℃.Quantification was performed in the negative ion multiple -reac-tion-monitoring ( MRM ) mode.Results Determination of nifedipine had good linearity in the concentration range of 0.03 -80 ng · mL-1.The lower limit of quantization was 0.03 ng · mL-1 , extraction recovery rate was from 94.71% to 102.3%, and the intra -day and inter -day RSD were both less than 9.8%.Conclusion This method is specific , simple, sensitive, rapid, accurate and suitable for determination of nife-dipine in human plasma.