CAJ | 학술논문

目的：用大孔树脂分离纯化刺五加苷的2种主要成分作为控制刺五加药材质量的标准。方法用D-101大孔树脂对刺五加苷B、刺五加苷E进行分离纯化，用高效液相色谱法测定以上2个成分的含量，以此作为刺五加的质量标准。结果上样液浓度0.5 g· mL-1，吸附流速2 BV· h-1，30％乙醇的洗脱流速1 BV· h-1。透析袋处理。色谱柱：C18（4.6 mm ×250 mm，5μm）；检测波长220 nm；流动相：水-乙腈（0～10 min，90∶10；10～30 min，90～80∶10～20）；流速：1.0 mL· min-1；柱温25℃。结论该方法操作性强，重现性好，可作为刺五加药材质量控制的有效方法。
목적：용대공수지분리순화자오가감적2충주요성분작위공제자오가약재질량적표준。방법용D-101대공수지대자오가감B、자오가감E진행분리순화，용고효액상색보법측정이상2개성분적함량，이차작위자오가적질량표준。결과상양액농도0.5 g· mL-1，흡부류속2 BV· h-1，30％을순적세탈류속1 BV· h-1。투석대처리。색보주：C18（4.6 mm ×250 mm，5μm）；검측파장220 nm；류동상：수-을정（0～10 min，90∶10；10～30 min，90～80∶10～20）；류속：1.0 mL· min-1；주온25℃。결론해방법조작성강，중현성호，가작위자오가약재질량공제적유효방법。
Objective To purification the Acanthopanax glycosides of two kinds of main component with macroporous resin method to be control the standard of quality of Acanthopanax medicinal materials.Methods Eleutheroside B and Eleutheroside E were isolated and purified by macro-porous resin.Determination of Acanthopanax senticosus Harms of Eleuth-eroside B and Eleutheroside E content by HPLC , as a quality standard of Acanthopanax senticosus.Results The sample concentration is 0.5 g· mL-1, adsorption velocity 2 BV· h-1, 30% ethanol eluent, elution rate 1 BV· h-1.Dialysis treatment.Column:C18 (4.6 mm ×250 mm,5μm);detection wavelength was 220 nm;mobile phase:water-acetoni-trile (0~10 min, 90∶10;10~30 min,90~80∶10~20 );flow rate:1.0 mL· min -1; Column temperature was 25 ℃.Conclusion The method of operation has good reproducibility which can be used as an effective method to control the quality of Acanthopanax senticosus harms.