CAJ | 학술논문

目的：观察骨化三醇联合顺铂对人子宫内膜癌细胞株（ HEC-1-A）裸鼠移植瘤的抑制作用。方法将HEC-1-A细胞接种于裸鼠右侧背部皮下，并将成瘤的裸鼠分为对照组（0.9％NaCl 0.25 mL）、骨化三醇组（0.05μg）、顺铂组（50μg）、骨化三醇联合顺铂组（0.05μg＋50μg），每周处理1次，连用5周。结果骨化三醇组、顺铂组和联合用药组与对照组比较，移植瘤体积均明显减小（ P＜0.05）；同时，Bcl -2 mRNA和Bax mRNA 表达强度差异均有统计学意义（P＜0.05）。联合用药组移植瘤凋亡率显著高于其他3组（P＜0.05）。结论骨化三醇对HEC-1-A移植瘤具有较强的抗肿瘤作用，其机制可能与Bax/Bcl-2途径诱导HEC-1-A肿瘤细胞凋亡有关。
목적：관찰골화삼순연합순박대인자궁내막암세포주（ HEC-1-A）라서이식류적억제작용。방법장HEC-1-A세포접충우라서우측배부피하，병장성류적라서분위대조조（0.9％NaCl 0.25 mL）、골화삼순조（0.05μg）、순박조（50μg）、골화삼순연합순박조（0.05μg＋50μg），매주처리1차，련용5주。결과골화삼순조、순박조화연합용약조여대조조비교，이식류체적균명현감소（ P＜0.05）；동시，Bcl -2 mRNA화Bax mRNA 표체강도차이균유통계학의의（P＜0.05）。연합용약조이식류조망솔현저고우기타3조（P＜0.05）。결론골화삼순대HEC-1-A이식류구유교강적항종류작용，기궤제가능여Bax/Bcl-2도경유도HEC-1-A종류세포조망유관。
Objective To investigate the effect calcitriol combinating cisplatin on the human endometrial cancer ( HEC -1-A) transplanta-tion tumor of athymic mouse.Methods The HEC-1-A cell line was cultured in vitro.The thymic mouse with successfully tumor burden were divide into four groups:the control group (0.9%NaCl 0.25 mL), cal-citriol group (0.05 μg) , cisplatin group (50 μg) and combinating ( cal-citriol plus cisplatin ) therapy group.All of the four groups were treated once a week until 5 weeks.Results The difference of transplantation tumor volume between three therapy groups and control group is signifi-cant ( P<0.05 ).The apoptosis rate of the control , cisplati , calcitriol and combinating groups were ( 1.45 ±0.23 )%, ( 25.62 ±1.16 )%, (22.52 ±1.25 )%and (31.59 ±1.93 )%, respectively.There was sig-nificant statistical difference between combination group with the other three groups ( P<0.05 ).The mRNA expression level of Bcl -2 and Bax among the three therapy groups compared with control group were signifi-cant ( P <0.05 ).Conclusion Calcitriol had a strong anti -tumor effect in HEC-1-A transplantation tumor , the anti-tumor mechanism may associate with the Bax/Bcl-2 pathway induced HEC -1-A tumor cell apoptosis.