CAJ | 학술논문

目的：探讨血管生成素-1、血管生成素-2（ Ang-1/2）和血管内皮生长因子（ VEGF）在子宫颈鳞状细胞癌（ SCC）组织中的表达及与放化疗相关性。方法入选49例单纯手术治疗的子宫颈鳞状细胞癌（ SCC-O ），25例术前经过化疗后再手术治疗者（ SCC-CO）以及18例术前经过放疗再手术治疗的子宫颈鳞状细胞癌（ SCC-RO）3组患者，用免疫组织化学染色法检测Ang-1、Ang-2及VEGF的表达，分析其与CD34标记的微血管密度（ MVD）及D2-40标记的微淋巴管密度（ LVD）的关系。结果从宫颈癌Ⅰ期到Ⅱ期Ang-2蛋白及VEGF蛋白表达强度有所增强，而 Ang -1蛋白的表达则下降；放、化疗使 Ang -2及VEGF蛋白表达强度明显降低（ P＜0.05），且使MVD值及LVD值显著降低（ P＜0.05）；Ang -2及 VEGF 表达强度与 MVD 值及 LVD 值呈显著正相关（ P ＜0.05）。结论 Ang-2及VEGF在子宫颈癌中的高表达与肿瘤进展有关；Ang-2与VEGF在促进宫颈癌血管及淋巴管的形成上起相互协同作用；放疗及化疗可明显抑制宫颈癌Ang-2与VEGF表达和血管及淋巴管的形成。
목적：탐토혈관생성소-1、혈관생성소-2（ Ang-1/2）화혈관내피생장인자（ VEGF）재자궁경린상세포암（ SCC）조직중적표체급여방화료상관성。방법입선49례단순수술치료적자궁경린상세포암（ SCC-O ），25례술전경과화료후재수술치료자（ SCC-CO）이급18례술전경과방료재수술치료적자궁경린상세포암（ SCC-RO）3조환자，용면역조직화학염색법검측Ang-1、Ang-2급VEGF적표체，분석기여CD34표기적미혈관밀도（ MVD）급D2-40표기적미림파관밀도（ LVD）적관계。결과종궁경암Ⅰ기도Ⅱ기Ang-2단백급VEGF단백표체강도유소증강，이 Ang -1단백적표체칙하강；방、화료사 Ang -2급VEGF단백표체강도명현강저（ P＜0.05），차사MVD치급LVD치현저강저（ P＜0.05）；Ang -2급 VEGF 표체강도여 MVD 치급 LVD 치정현저정상관（ P ＜0.05）。결론 Ang-2급VEGF재자궁경암중적고표체여종류진전유관；Ang-2여VEGF재촉진궁경암혈관급림파관적형성상기상호협동작용；방료급화료가명현억제궁경암Ang-2여VEGF표체화혈관급림파관적형성。
Objective To investigate the expression of angiopoietin -1 (Ang-1), angiopoietin -2 (Ang-2) and vascular endothelial growth factor (VEGF) in cervical carcinoma (SCC) tissues and relations with radiotherapy and chemotherapy.Methods Immunohistochemistry SP method was used to detect the expression of Ang -1 , 2 and VEGF in 3 groups:49 cases of cervical squamous cell carcinoma ( SCC-O) treated with simple treatment of operation , 25 subjects with chemotherapy treat-ment before operation ( SCC-CO) and 18 cases of cervical squamous cell carcinoma treated with preoperative radiotherapy and followed operation ( SCC-RO ).And the relations of those expressions with microvessel density CD34 positive markers ( MVD ) and D2 -40 marker -positive lymphatic vessel density ( LVD ) were analyzed.Results The expres-sion Ang-2 and VEGF were increased from stage I to II of cervical canc-er( P>0.05 ) , and the expression of Ang -1 protein was in the oppo-site.Radiotherapy and chemotherapy obviously decreased the expression of Ang-2 and VEGF ( P<0.05 ) , as well as both the MVD value and LVD value ( P<0.05 ).The expression of Ang-2 and VEGF were posi-tively correlated with the value of MVD and LVD in the tumor (P<0.05).Conclusion High expressions of Ang -1, Ang-2 and VEGF in cervical cancer tissue might play an important role in the tumor advancement.The expressions of Ang -2 and VEGF are synergic to improve generation of cervical blood and lymphatic vessels.Cervical cancer radiotherapy and chemotherapy could significantly inhibit the for-mation of blood vessels and lymphatic vessels in tumor tissue.