CAJ | 학술논문

目的：拟观察外源性神经生长因子对去神经大鼠血清BGP和TRACP的影响，探讨神经生长对废用性骨质疏松的预防作用。方法50只大鼠随机抽签法分为5组：假手术组（ SHAM ）、去神经组（ DN ）、神经生长因子组（ NGF ）、被动运动组（ PM）、神经生长因子与被动运动联合组（ NGF＋PM）。假手术组不切除坐骨神经和股神经，其他各组通过切除大鼠右侧坐骨神经和股神经建立SD大鼠右侧后肢的去神经模型，通过被动运动和外源性生长因子的干预措施，观察各组大鼠血清BGP和TRACP指标。结果与假手术组相比，去神经各组血清BGP方面明显降低（P＜0.01），血清TRACP明显升高（P ＜0.05）；与DN组相比， NGF组和NGF＋PM组与之都有显著性差异（P ＜0.05），PM与DN组之间虽无统计学意义（P ＞0.05）；与DN组血清TRACP相比，PM组和PM＋NGF组与之有显著性差异（P ＜0.05），NGF组与之无差异（P ＞0.05）；结论大鼠下肢缺乏神经支配导致肌肉萎缩、骨正常代谢遭到破坏，被动运动和神经生长因子能提高去神经大鼠血清BGP水平、降低血清TRACP的含量，一定程度上能预防去神经型废用性骨质疏松的发生。
목적：의관찰외원성신경생장인자대거신경대서혈청BGP화TRACP적영향，탐토신경생장대폐용성골질소송적예방작용。방법50지대서수궤추첨법분위5조：가수술조（ SHAM ）、거신경조（ DN ）、신경생장인자조（ NGF ）、피동운동조（ PM）、신경생장인자여피동운동연합조（ NGF＋PM）。가수술조불절제좌골신경화고신경，기타각조통과절제대서우측좌골신경화고신경건립SD대서우측후지적거신경모형，통과피동운동화외원성생장인자적간예조시，관찰각조대서혈청BGP화TRACP지표。결과여가수술조상비，거신경각조혈청BGP방면명현강저（P＜0.01），혈청TRACP명현승고（P ＜0.05）；여DN조상비， NGF조화NGF＋PM조여지도유현저성차이（P ＜0.05），PM여DN조지간수무통계학의의（P ＞0.05）；여DN조혈청TRACP상비，PM조화PM＋NGF조여지유현저성차이（P ＜0.05），NGF조여지무차이（P ＞0.05）；결론대서하지결핍신경지배도치기육위축、골정상대사조도파배，피동운동화신경생장인자능제고거신경대서혈청BGP수평、강저혈청TRACP적함량，일정정도상능예방거신경형폐용성골질소송적발생。
Objective To investigate the effect of exogenous NGF on the serum BGP and TRACP in denervated rats, and to explore the preventive effect of nerve growth on disuse osteoporosis.Methods Fifty rats were randomly divided into 5 groups:sham operation group ( SHAM) , denervation group ( DN) , NGF group ( NGF) , passive movement group ( PM) , and combination group ( NGF+PM) .Rats in SHAM group were injected with saline, without the excision of the sciatic nerve and the femoral nerve.Rats in other 4 groups were all induced disuse osteoporosis by the excision of the sciatic nerve and the femoral nerve.The serum levels of BGP and TRACP were observed.Results Compared with that in SHAM group, BGP level in the other 4 groups decreased significantly (P<0.01), but TRACP level increased significantly (P<0.05).Compared with that in DN group, BGP level in NGF group and NGF+PM group was significantly different (P<0.05), while no significant difference between PM group and DN group was observed ( P>0.05 ) .The serum TRACP level in PM group and PM+NGF group was significantly different with that in DN group (P<0.05), while no significant difference between NGF group and DN group was observed (P>0.05). Conclusion Denervation in rat lower limbs leads to muscular atrophy and destroyed bone metabolism .Passive movement and nerve growth factor can improve the serum BGP level and reduce the serum TRACP level in denervated rats , showing a preventive effect on the disuse osteoporosis to a certain extent .