CAJ | 학술논문

目的：探讨常规体外受精（IVF）及卵胞浆内单精子注射（ICSI）后单原核胚胎的发育潜能，为提高卵子利用率探寻可行的方法。方法：回顾性分析3614个IVF周期和1306个ICSI周期的患者资料。按照年龄分为＜30岁、30～35岁和＞35岁3组，分析不同授精方式下女方年龄对单原核受精卵出现的影响，并进一步研究35岁以下患者中，不同授精方式对单原核胚胎发育潜能的影响。结果：在IVF和ICSI 周期中，＞35岁组的单原核受精卵形成率显著高于＜30岁组（P＜0.05）。35岁以下女性患者中，IVF组单原核胚胎的囊胚形成率及优质囊胚形成率显著高于ICSI组（28.97％vs 12.12％，11.90％vs 0），并具有统计学差异（P＜0.05）。结论：女方年龄、授精方式可能与异常受精的发生有关。在行辅助生殖技术（ART）时应综合考虑这些因素，为临床提高卵子利用率提供新思路。
목적：탐토상규체외수정（IVF）급란포장내단정자주사（ICSI）후단원핵배태적발육잠능，위제고란자이용솔탐심가행적방법。방법：회고성분석3614개IVF주기화1306개ICSI주기적환자자료。안조년령분위＜30세、30～35세화＞35세3조，분석불동수정방식하녀방년령대단원핵수정란출현적영향，병진일보연구35세이하환자중，불동수정방식대단원핵배태발육잠능적영향。결과：재IVF화ICSI 주기중，＞35세조적단원핵수정란형성솔현저고우＜30세조（P＜0.05）。35세이하녀성환자중，IVF조단원핵배태적낭배형성솔급우질낭배형성솔현저고우ICSI조（28.97％vs 12.12％，11.90％vs 0），병구유통계학차이（P＜0.05）。결론：녀방년령、수정방식가능여이상수정적발생유관。재행보조생식기술（ART）시응종합고필저사인소，위림상제고란자이용솔제공신사로。
Objectives:To investigate the developmental potential of monopronucleus embryos in in-vitro fertilization (IVF)and intracytoplasmic sperm injection (ICSI)to explore a feasible approach for improving the uti-lization rate of oocytes.Method:Data of 3614 patients in IVF cycles and 1306 patients in ICSI cycles were retro-spectively analyzed respectively,and divided into three groups according to age of patients,<30 years group,30~35 years group,and >35 years group.The impact of age on monopronucleus zygotes was analyzed in different ferti-lization modes and the influence of different fertilization modes on the developmental potential of monopronucleus zy-gotes was further studied in group of patients under 35 years old.Results:The formation rate of monopronucleus zy-gotes for the group >35 years was substantially higher than that in group <30 years (P<0.05 )in IVF and ICSI cycles.Among female patients under 35 years old,the blastocyst rate and high-quality blastocyst rate of embryos from monopronucleus zygotes in the IVF group were substantially higher than the ICSI group (28.97%vs 12.12%, 1 1.90% vs 0),with statistically significant differences (P<0.05 ).Conclusion:The age of female patients and fertilization modes may be related to the occurrence of abnormal fertilization.Multiple factors should be considered during assisted reproductive technology (ART),to provide new ideas for improving the utilization of oocytes.