中国性科学
中國性科學
중국성과학
THE CHINESE JOURNAL OF HUMAN SEXUALITY
2014年
6期
29-32
,共4页
宫颈癌%蛋白指纹%诊断
宮頸癌%蛋白指紋%診斷
궁경암%단백지문%진단
Cervical cancer%Protein fingerprinting%Diagnosis
目的:探讨蛋白指纹图谱技术筛选宫颈癌患者血清中可用于诊断的特异性蛋白标志物。方法:采用弱阳离子纳米磁珠微球捕获的蛋白,用PBSⅡ-C型蛋白芯片阅读仪检测绘制成蛋白指纹图谱。所有蛋白指纹图谱采用Bomarker Wizard软件分析之后,用Biomarker Pattern软件识别最终用于诊断的蛋白标志物,并优化组合建立诊断模型。结果:在宫颈癌与对照组之间找到74个差异蛋白峰(P<0.01),其中质荷比(m/z)为3947 Da,4175 Da,5906 Da的蛋白峰组成的诊断模型可以将宫颈癌患者和健康志愿者区分,敏感性达94.0%(47/50),特异性为90.8%(59/65)。经双盲实验验证,该模型对宫颈癌诊断的敏感性达90.5%(38/42),特异性为96.0%(48/50)。结论:MALDI-TOF-MS技术联合纳米磁珠可以用来检测宫颈癌血清中的特异性蛋白标志物,并建立敏感性和特异性均较高的宫颈癌诊断模型。
目的:探討蛋白指紋圖譜技術篩選宮頸癌患者血清中可用于診斷的特異性蛋白標誌物。方法:採用弱暘離子納米磁珠微毬捕穫的蛋白,用PBSⅡ-C型蛋白芯片閱讀儀檢測繪製成蛋白指紋圖譜。所有蛋白指紋圖譜採用Bomarker Wizard軟件分析之後,用Biomarker Pattern軟件識彆最終用于診斷的蛋白標誌物,併優化組閤建立診斷模型。結果:在宮頸癌與對照組之間找到74箇差異蛋白峰(P<0.01),其中質荷比(m/z)為3947 Da,4175 Da,5906 Da的蛋白峰組成的診斷模型可以將宮頸癌患者和健康誌願者區分,敏感性達94.0%(47/50),特異性為90.8%(59/65)。經雙盲實驗驗證,該模型對宮頸癌診斷的敏感性達90.5%(38/42),特異性為96.0%(48/50)。結論:MALDI-TOF-MS技術聯閤納米磁珠可以用來檢測宮頸癌血清中的特異性蛋白標誌物,併建立敏感性和特異性均較高的宮頸癌診斷模型。
목적:탐토단백지문도보기술사선궁경암환자혈청중가용우진단적특이성단백표지물。방법:채용약양리자납미자주미구포획적단백,용PBSⅡ-C형단백심편열독의검측회제성단백지문도보。소유단백지문도보채용Bomarker Wizard연건분석지후,용Biomarker Pattern연건식별최종용우진단적단백표지물,병우화조합건립진단모형。결과:재궁경암여대조조지간조도74개차이단백봉(P<0.01),기중질하비(m/z)위3947 Da,4175 Da,5906 Da적단백봉조성적진단모형가이장궁경암환자화건강지원자구분,민감성체94.0%(47/50),특이성위90.8%(59/65)。경쌍맹실험험증,해모형대궁경암진단적민감성체90.5%(38/42),특이성위96.0%(48/50)。결론:MALDI-TOF-MS기술연합납미자주가이용래검측궁경암혈청중적특이성단백표지물,병건립민감성화특이성균교고적궁경암진단모형。
Objectives:To screen the potential protein biomarkers in serum for the diagnosis of cervical cancer by protemic fingerprint.Methods:Proteomic fingerprint combining magnetic beads with MALDI-TOF-MS was used to profile and compare the serum proteins of patients with cervical cancer and healthy volunteers.PBSII-C protein chips reader was used to analyze protein peaks for significant difference.The protein fingerprint expression of all the serum samples and the resulting profiles between cancer patients and volunteers were analyzed with Bio-marker Wizard system.Results:Seventy-four differentially expressed proteins in serum were screened between pa-tients with cervical cancer and health individuals (P<0.01).Proteins (3974 Da,4175 Da,5906 Da)were used for building a clinprot model which was able to classify patients with cervical cancer and health individuals with a sensitivity of 94.0%(47/50)and a specificity of 90.8%(59/65).Blind test data indicated a sensitivity of 90.5%(38/42)and a specificity of 96.0%(48/50).Conclusion:The biomarks in serum for cervical cancer can be dis-covered by MALDI-TOF-MS combining with magnetic beads,which is a powerful and reliable diagnostic method for cervical cancer with a high sensitivity and specificity.