CAJ | 학술논문

目的：观察宫颈上皮内瘤样病变（ CIN）及宫颈癌（ CC）组织中c-Jun氨基末端激酶（研究JNK）和信号转导及转录活化因子3（STAT3）的表达变化，并探讨其意义。方法采用免疫组化链霉素抗生物素蛋白-生物素-过氧化物酶（SABC）法，检测10例正常宫颈组织（NCE）、105例CIN和66例宫颈癌（CC）组织中JNK和STAT3蛋白表达情况，并分析其阳性表达率与CC临床病理参数的关系。结果 JNK蛋白在NCE、CIN及CC组织中阳性表达率分别为10．0％、45．7％、75．8％，三者两两相比，P均＜0．05。 STAT3蛋白在NCE、CIN及CC组织中阳性表达率分别为10．0％、48．6％、83．3％，三者两两相比，P均＜0．05。 JNK、STAT3蛋白阳性表达均与CC患者年龄、临床分期、淋巴结转移无关，但与肿瘤组织学分级有关，其中低分化癌组织中JNK、STAT3蛋白水平表达显著高于高、中分化癌组织（P＜0．005）。结论 JNK、STAT3蛋白在CIN及CC组织中表达增强，与CC发生、发展相关；检测两者表达水平可作为CC预后判断的一项客观指标及为个体化治疗提供依据。
목적：관찰궁경상피내류양병변（ CIN）급궁경암（ CC）조직중c-Jun안기말단격매（연구JNK）화신호전도급전록활화인자3（STAT3）적표체변화，병탐토기의의。방법채용면역조화련매소항생물소단백-생물소-과양화물매（SABC）법，검측10례정상궁경조직（NCE）、105례CIN화66례궁경암（CC）조직중JNK화STAT3단백표체정황，병분석기양성표체솔여CC림상병리삼수적관계。결과 JNK단백재NCE、CIN급CC조직중양성표체솔분별위10．0％、45．7％、75．8％，삼자량량상비，P균＜0．05。 STAT3단백재NCE、CIN급CC조직중양성표체솔분별위10．0％、48．6％、83．3％，삼자량량상비，P균＜0．05。 JNK、STAT3단백양성표체균여CC환자년령、림상분기、림파결전이무관，단여종류조직학분급유관，기중저분화암조직중JNK、STAT3단백수평표체현저고우고、중분화암조직（P＜0．005）。결론 JNK、STAT3단백재CIN급CC조직중표체증강，여CC발생、발전상관；검측량자표체수평가작위CC예후판단적일항객관지표급위개체화치료제공의거。
Objective To observe the expression of c-Jun N-terminal kinase ( JNK) and signal transducer and activator of transcription3(STAT3) in cervical intraepithelial neoplasia(CIN) and cervical cancer(CC) tissues and to explore its signifi-cance .Methods Expression of JNK and STAT 3 protein was detected by immunohistochemical SABC method in 10 cases of nor-mal cervical epithelium(NCE),105 cases of cervical intraepithelial neoplasia(CIN) and 66 cases of cervical cancer(CC),their relationship with clinicopathological parameters of cervical cancer were analyzed .Results The positive expression rates of JNK protein in NCE,CIN and CC tissue were 10.0%,45.7%and 75.8%respectively.There was significant difference between these groups (P<0.05).Meanwhile,the positive expression rates of STAT3 protein NCE,CIN and CC tissue were 10.0%,48.6%and 83.3%respectively.There was significant difference between these groups (P<0.05).The JNK and STAT3 expression was not related to age,clinical stage and lymph node metastasis ,but related to histological grade .The expression of JNK ans STAT3 was higher in poorly differentiated carcinoma than in highly-and moderately-differentiated one,(P<0.005,χ2 =12.08,12.61).Con-clusion The expression of JNK and STAT3 protein in cervical intraepithelial neoplasia and cervical cancer increase and correlate with the carcinogenesis ,development of cervical cancer .Detecting JNK and STAT3 protein expression may be efficient indicators of predicting prognosis of cervical cancer and have important implications for individual treatment .