CAJ | 학술논문

目的：探讨补肾益髓生血法再生障碍性贫血（Aplastic Anemia，AA，简称再障）大鼠含药血清对大鼠骨髓造血干细胞红系分化JAK2/STAT5信号通路的影响。方法：在前期整体动物实验的基础上，体外培养正常大鼠造血干细胞，诱导其定向红系分化，分为空白对照组、正常对照组、模型组、司坦唑醇组（康力龙组）、益髓生血组、温肾生血组和滋肾生血组，在培养体系中加入含药血清干预4d后，提取红系细胞总RNA及蛋白，分别采用RT-PCR和Western blot检测细胞JAK2、STAT5mRNA及蛋白表达情况。结果：与正常对照组相比，模型组细胞JAK2、STAT5mRNA及蛋白表达均明显降低（P＜0.01）；与模型组相比，各治疗组JAK2、STAT5mRNA及蛋白表达明显升高（P＜0.01或P＜0.05）；滋肾生血组JAK2、STAT5mRNA及蛋白表达高于益髓生血组和温肾生血组（P＜0.05）。结论：补肾益髓生血法能够增强JAK2、STAT5的表达，可能通过影响JAK2/STAT5信号通路来促进红系细胞的分化成熟，其中滋肾生血法优于益髓生血法和温肾生血法。
목적：탐토보신익수생혈법재생장애성빈혈（Aplastic Anemia，AA，간칭재장）대서함약혈청대대서골수조혈간세포홍계분화JAK2/STAT5신호통로적영향。방법：재전기정체동물실험적기출상，체외배양정상대서조혈간세포，유도기정향홍계분화，분위공백대조조、정상대조조、모형조、사탄서순조（강력룡조）、익수생혈조、온신생혈조화자신생혈조，재배양체계중가입함약혈청간예4d후，제취홍계세포총RNA급단백，분별채용RT-PCR화Western blot검측세포JAK2、STAT5mRNA급단백표체정황。결과：여정상대조조상비，모형조세포JAK2、STAT5mRNA급단백표체균명현강저（P＜0.01）；여모형조상비，각치료조JAK2、STAT5mRNA급단백표체명현승고（P＜0.01혹P＜0.05）；자신생혈조JAK2、STAT5mRNA급단백표체고우익수생혈조화온신생혈조（P＜0.05）。결론：보신익수생혈법능구증강JAK2、STAT5적표체，가능통과영향JAK2/STAT5신호통로래촉진홍계세포적분화성숙，기중자신생혈법우우익수생혈법화온신생혈법。
Objective:To investigate the effect of tonifying kidney,benefiting marrow and engendering blood method on JAK2/STAT5 signaling pathway in the erythroid differentiation of hematopoietic stem cells.Methods:On the basis of the previous in vivo animal ex-periment,the hematopoietic stem cells were cultivated with AA rats serum and induced erythroid differentiation in vitro.The cells were divided into the following seven groups:blank control group,normal control group,model group,stanozolol group,benefiting marrow and engendering blood group,warming kidney and engendering blood group,nourishing kidney and engendering blood group.Erythroid cells RNA and protein were extracted in 4 days respectively,and the level of JAK2,STAT5 mRNA and protein in erythroid cells were detected with RT-PCR and Western blot.Results:Compared with the normal control group,the expressing of JAK2,STAT5 mRNA and protein in cells decreased significantly in the model group(P<0.01).Compared with the model group,the expression of JAK2,STAT5 mRNA and protein both significantly increased in treatment groups(P<0.01 or P<0.05),while the nourishing kidney group was significantly better than the benefiting marrow group and warming kidney group of the expression of JAK2,STAT5mRNA and protein.Conclusion:The method of tonifying kidney,benefiting marrow and engendering blood can increase the expression of JAK2,STAT5 and promote erythroid differentiation and maturation by affecting JAK2/STAT5 signaling pathway and stimulating activity.The nourishing kidney and engende-ring blood method is better than benefiting marrow and warming kidney method.