现代养生B
現代養生B
현대양생B
HEALTH CARE TODAY
2014年
6期
31-31,32
,共2页
李炳东%李国萍%于开军%周德鹏
李炳東%李國萍%于開軍%週德鵬
리병동%리국평%우개군%주덕붕
高效液相色谱法%苯妥英钠%苯巴比妥%血药浓度
高效液相色譜法%苯妥英鈉%苯巴比妥%血藥濃度
고효액상색보법%분타영납%분파비타%혈약농도
HPLC%phenytoin sodium%phenobarbit%Blood drug concentration
目的：建立测定人血清中苯妥英钠浓度的方法。方法：采用大连依利特HypersilODS2色谱柱(4.6mm×150mm,5μm )，流动相为甲醇-乙腈-水（20∶15∶65），流速为1mL·min-1，检测波长为210nm，柱温为30℃，内标为苯巴比妥。结果：苯妥英钠血药浓度在6.25～100 mg·L-1范围内线性关系良好(r=0.9995，n=6)，最低检测浓度为1.56mg·L-1；平均回收率为99.64%～100.2%，日内与日间RSD均<5%(n=5)。结论：本方法快速、简便、准确，适用于临床苯妥英钠的血药浓度监测。
目的：建立測定人血清中苯妥英鈉濃度的方法。方法：採用大連依利特HypersilODS2色譜柱(4.6mm×150mm,5μm )，流動相為甲醇-乙腈-水（20∶15∶65），流速為1mL·min-1，檢測波長為210nm，柱溫為30℃，內標為苯巴比妥。結果：苯妥英鈉血藥濃度在6.25～100 mg·L-1範圍內線性關繫良好(r=0.9995，n=6)，最低檢測濃度為1.56mg·L-1；平均迴收率為99.64%～100.2%，日內與日間RSD均<5%(n=5)。結論：本方法快速、簡便、準確，適用于臨床苯妥英鈉的血藥濃度鑑測。
목적：건립측정인혈청중분타영납농도적방법。방법：채용대련의리특HypersilODS2색보주(4.6mm×150mm,5μm )，류동상위갑순-을정-수（20∶15∶65），류속위1mL·min-1，검측파장위210nm，주온위30℃，내표위분파비타。결과：분타영납혈약농도재6.25～100 mg·L-1범위내선성관계량호(r=0.9995，n=6)，최저검측농도위1.56mg·L-1；평균회수솔위99.64%～100.2%，일내여일간RSD균<5%(n=5)。결론：본방법쾌속、간편、준학，괄용우림상분타영납적혈약농도감측。
Objective:To establish the method for the concentration determination of phenytoin sodium in human serum. Methods: The chromatographic column was Hypersil ODS2 (150 mm×4.6 mm,5 μm) column with mobile phase consisted of methanol-acetonitrile-water (20:15:65) at flow rate of 1.0 mL?min-1. The colum temperature was 30℃ and the detection wavelength was set at 210 nm. The phenobarbit was used as internal standard. Results: Blood concentrations of phenytoin was within the detection ranges of 6.25 ~ 100 mg?L-1(r=0.999 5,n=6).The ratios of average recovery was 99.64% ~ 100.2% and precision within a day and among days were less than 5.0%.Conclusion: The method is rapid, simple, accurate and suitable for routine monitoring of clinical need.
