CAJ | 학술논문

目的分析三阴性乳腺癌( trip nenegative breast cancer, TNBC)患者外周血及病灶微环境辅助性T细胞和调节性T细胞的表达水平。方法选择2012年1-4月经临床及病理确诊的女性TNBC 34例( TNBC组)、非三阴性乳腺癌(NTNBC)39例(NTNBC组)及同期确诊为乳腺良性疾病女性患者27例(对照组),均检测外周血及病灶微环境辅助性 T 细胞( CD3+、CD4+、CD8+、CD4+/CD8+)和调节性 T 细胞( CD4CD25+ Treg 和 CD4+CD25+Foxp3+Treg),并进行比较。结果 TNBC组外周血及病灶微环境CD3+、CD4+水平、CD4+/CD8+比值均明显低于NTNBC及对照组,而CD8+水平则明显高于其他两组(P<0.05)；TNBC组外周血及病灶微环境CD4CD25+ Treg及CD4+CD25+Foxp3+ Treg表达水平均明显高于NTNBC组及对照组( P<0.05)。结论 TNBC患者存在明确的外周血及病灶微环境调节性T细胞和辅助性T细胞异常表达,其可作为本病免疫学病因诊断及评估疗效的特征性标识,并为今后寻找TNBC免疫治疗途径提供试验数据。
목적분석삼음성유선암( trip nenegative breast cancer, TNBC)환자외주혈급병조미배경보조성T세포화조절성T세포적표체수평。방법선택2012년1-4월경림상급병리학진적녀성TNBC 34례( TNBC조)、비삼음성유선암(NTNBC)39례(NTNBC조)급동기학진위유선량성질병녀성환자27례(대조조),균검측외주혈급병조미배경보조성 T 세포( CD3+、CD4+、CD8+、CD4+/CD8+)화조절성 T 세포( CD4CD25+ Treg 화 CD4+CD25+Foxp3+Treg),병진행비교。결과 TNBC조외주혈급병조미배경CD3+、CD4+수평、CD4+/CD8+비치균명현저우NTNBC급대조조,이CD8+수평칙명현고우기타량조(P<0.05)；TNBC조외주혈급병조미배경CD4CD25+ Treg급CD4+CD25+Foxp3+ Treg표체수평균명현고우NTNBC조급대조조( P<0.05)。결론 TNBC환자존재명학적외주혈급병조미배경조절성T세포화보조성T세포이상표체,기가작위본병면역학병인진단급평고료효적특정성표식,병위금후심조TNBC면역치료도경제공시험수거。
Objective To analyze the levels of the auxiliary and regulatory T cells expression in peripheral blood and lesion microenvironment of patients with triple-negative breast cancer ( TNBC ) . Methods The levels of auxiliary T cells (CD3 +, CD4 +, CD8 +, CD4 + /CD8 +) and regulatory T cells (CD4CD25 + Treg and CD4 + CD25 + Foxp3 + Treg) in periph-eral blood and lesion microenvironment were detected for 34 TNBC patients ( TNBC group) confirmed by clinical and patholog-ical results, 39 non-TNBC patients ( NTNBC group) and 27 patients with benign breast diseases ( control group) from 2012 January to April, and the levels in the three groups were also compared. Results In the TNBC group, the levels of CD3 +, CD4 + and CD4 + /CD8 + were significantly lower than those in the NTNBC and control groups, but the CD8 + level was signifi-cantly higher than that in the NTNBC and control groups (P <0.05); the levels of CD4CD25 + Treg and CD4 + CD25 +Foxp3 + Treg in the TNBC group were significantly higher than those in the NTNBC and control groups (P<0.05). Conclu-sion The auxiliary and regulatory T cells are abnormally expressed in the peripheral blood and lesion microenvironment in TNBC patients, which is a characteristic parameter of immunology in etiological diagnosis and evaluation effect, wihich may provide test data to find a method of TNBC immunotherapy in the future.