CAJ | 학술논문

目的：研究人牙囊细胞成骨分化前后LIM矿化蛋白-1的表达。探讨LIM矿化蛋白-1在牙囊细胞向牙周组织分化过程中的作用。方法：组织块加酶消化法分离培养人牙囊细胞，实验组培养液中加入矿化诱导剂，对照组不加矿化诱导剂。培养4周后，免疫细胞化学检测骨涎蛋白的表达；Von Kossa染色检测矿化结节的形成。矿化诱导前后，采用RT-PCR技术检测LIM矿化蛋白-1。结果：培养4周，实验组矿化结节形成，骨涎蛋白免疫细胞化学呈阳性表达， Von Kossa染色阳性；对照组未形成矿化结节，骨涎蛋白表达弱阳性，Von Kossa染色阴性。 RT-PCR结果显示LIM矿化蛋白-1在实验组强阳性表达，对照组弱表达。结论：LIM矿化蛋白-1与胚胎期人牙囊细胞的体外矿化过程相关，提示LIM矿化蛋白-1可能在胚胎期牙囊细胞的分化、矿化中发挥一定作用。
목적：연구인아낭세포성골분화전후LIM광화단백-1적표체。탐토LIM광화단백-1재아낭세포향아주조직분화과정중적작용。방법：조직괴가매소화법분리배양인아낭세포，실험조배양액중가입광화유도제，대조조불가광화유도제。배양4주후，면역세포화학검측골연단백적표체；Von Kossa염색검측광화결절적형성。광화유도전후，채용RT-PCR기술검측LIM광화단백-1。결과：배양4주，실험조광화결절형성，골연단백면역세포화학정양성표체， Von Kossa염색양성；대조조미형성광화결절，골연단백표체약양성，Von Kossa염색음성。 RT-PCR결과현시LIM광화단백-1재실험조강양성표체，대조조약표체。결론：LIM광화단백-1여배태기인아낭세포적체외광화과정상관，제시LIM광화단백-1가능재배태기아낭세포적분화、광화중발휘일정작용。
Objective:To investigate Expression of LIM mineralization protein-1 after osteogenic differentiation of hu-man dental follicle cells compared with undifferented. Method:Human dental follicle cells were cultured in the presence of dexamethasone,asorbic acid and B-glycerophosphate for bone-like mineralized nodule formation ,characterized by Von Kos-sa staining and immunocyto chemistry of bone sialoprotein.Expression of LIM mineralization protein-1 was analzed by re-verse transcriptase-polymerase chain reaction. Result:LIM mineralization protein-1 were expressed both before mineraliza-tion and after,generally held at astatistically significantly higher level compared with the control. Conclusion:LIM mineral-ization protein-1 is found to be involved in the mineralization of human dental follicle cells.LIM mineralization protein-1 may play a role in the diferentiation and mineralization of human dental follicle cells.