CAJ | 학술논문

目的：探讨白细胞介素-lβ（IL-lβ）和肿瘤坏死因子-α（TNF-α）对牙周膜细胞内白血病抑制因子（LIF）表达的影响。方法：体外分离培养鉴定人牙周韧带细胞（HPDLC），取第3代细胞用于实验，利用牙周改建中高表达因子TNF-α和IL-1β刺激HPDLC后，实时定量反转录-聚合酶链反应检测LIF mRNA的表达。结果：IL-1β在浓度为0．1 ng/mL和5 ng/mL时，均显著促进LIF的分泌（P ＜0．01）。TNF-α在浓度为10 ng/mL时，明显促进LIF的分泌（P ＜0．05）。结论：牙周改建中高表达细胞因子IL-1β和TNF-α可促进牙周膜细胞中LIF的表达。
목적：탐토백세포개소-lβ（IL-lβ）화종류배사인자-α（TNF-α）대아주막세포내백혈병억제인자（LIF）표체적영향。방법：체외분리배양감정인아주인대세포（HPDLC），취제3대세포용우실험，이용아주개건중고표체인자TNF-α화IL-1β자격HPDLC후，실시정량반전록-취합매련반응검측LIF mRNA적표체。결과：IL-1β재농도위0．1 ng/mL화5 ng/mL시，균현저촉진LIF적분비（P ＜0．01）。TNF-α재농도위10 ng/mL시，명현촉진LIF적분비（P ＜0．05）。결론：아주개건중고표체세포인자IL-1β화TNF-α가촉진아주막세포중LIF적표체。
Objective:To investigate the effects of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) on the expression of leukemia inhibitory factor (LIF)mRNA in the human periodontal ligament cells (HPDLCs). Method:Human periodntal tissue was obtained from the extracted healthy premolar for orthodontic reasons. The HPDLCs used in this study underwent three passages. The expression of LIF mRNA in HPDLCs with or wihout IL-1β and TNF-α. Treatment was determined by realtime RT-PCR. Result:The expression of LIF mRNA was significantly induced by IL-1βin both 0.1 ng/mL and 5 ng/mL (P <0.01). The expression of LIF mRNA was markedly enhanced by TNF-α(P <0.05). Conclusion:Cytokines such as TNF-α and IL-1β highly expressed during periodontium remolding which dramatically stimulated the secretion of LIF in HPDLCs.