临床肿瘤学杂志
臨床腫瘤學雜誌
림상종류학잡지
CHINESE CLINICAL ONCOLOGY
2014年
6期
508-511
,共4页
6-磷酸果糖激酶-2/果糖双磷酸酶-2同工酶3%肝细胞癌%逆转录-聚合酶链反应%预后
6-燐痠果糖激酶-2/果糖雙燐痠酶-2同工酶3%肝細胞癌%逆轉錄-聚閤酶鏈反應%預後
6-린산과당격매-2/과당쌍린산매-2동공매3%간세포암%역전록-취합매련반응%예후
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase( PFKFB3)%Hepatocellular carcinoma%Reverse tran-scription-polymerase chain reaction( RT-PCR)%Prognosis
目的：探讨6-磷酸果糖激酶-2/果糖双磷酸酶-2同工酶3（ PFKFB3）在肝细胞癌（ HCC）组织中的表达及其与临床病理特征的关系。方法采用逆转录-聚合酶链反应（ RT-PCR）检测PFKFB3在42例HCC组织及其对应癌旁组织中的表达情况，并研究其与临床病理参数（性别、年龄、肿瘤直径、淋巴转移、静脉癌栓、治疗前甲胎蛋白、分化程度及肝内转移）间的关系，采用Kaplan-Meier法分析PFKFB3 mRNA表达对HCC患者生存的影响。结果 HCC和癌旁组织中PFKFB3 mRNA的阳性表达率分别为73?81％（31/42）和52?38％（22/42），相对表达量为1?075±0?171和0?877±0?451，HCC组织中 PFKFB3 mRNA的阳性表达率及相对表达量均高于癌旁组织（ P＜0?05）；PFKFB3 mRNA的表达与肿瘤TNM分期、分化程度、肝内转移及总生存期有关（ P＜0?05）。结论 PFKFB3在HCC的发生发展中发挥作用，且影响肝癌的分化、转移并提示肝癌患者预后不佳；可作为评估HCC恶性程度的生物学指标。
目的：探討6-燐痠果糖激酶-2/果糖雙燐痠酶-2同工酶3（ PFKFB3）在肝細胞癌（ HCC）組織中的錶達及其與臨床病理特徵的關繫。方法採用逆轉錄-聚閤酶鏈反應（ RT-PCR）檢測PFKFB3在42例HCC組織及其對應癌徬組織中的錶達情況，併研究其與臨床病理參數（性彆、年齡、腫瘤直徑、淋巴轉移、靜脈癌栓、治療前甲胎蛋白、分化程度及肝內轉移）間的關繫，採用Kaplan-Meier法分析PFKFB3 mRNA錶達對HCC患者生存的影響。結果 HCC和癌徬組織中PFKFB3 mRNA的暘性錶達率分彆為73?81％（31/42）和52?38％（22/42），相對錶達量為1?075±0?171和0?877±0?451，HCC組織中 PFKFB3 mRNA的暘性錶達率及相對錶達量均高于癌徬組織（ P＜0?05）；PFKFB3 mRNA的錶達與腫瘤TNM分期、分化程度、肝內轉移及總生存期有關（ P＜0?05）。結論 PFKFB3在HCC的髮生髮展中髮揮作用，且影響肝癌的分化、轉移併提示肝癌患者預後不佳；可作為評估HCC噁性程度的生物學指標。
목적：탐토6-린산과당격매-2/과당쌍린산매-2동공매3（ PFKFB3）재간세포암（ HCC）조직중적표체급기여림상병리특정적관계。방법채용역전록-취합매련반응（ RT-PCR）검측PFKFB3재42례HCC조직급기대응암방조직중적표체정황，병연구기여림상병리삼수（성별、년령、종류직경、림파전이、정맥암전、치료전갑태단백、분화정도급간내전이）간적관계，채용Kaplan-Meier법분석PFKFB3 mRNA표체대HCC환자생존적영향。결과 HCC화암방조직중PFKFB3 mRNA적양성표체솔분별위73?81％（31/42）화52?38％（22/42），상대표체량위1?075±0?171화0?877±0?451，HCC조직중 PFKFB3 mRNA적양성표체솔급상대표체량균고우암방조직（ P＜0?05）；PFKFB3 mRNA적표체여종류TNM분기、분화정도、간내전이급총생존기유관（ P＜0?05）。결론 PFKFB3재HCC적발생발전중발휘작용，차영향간암적분화、전이병제시간암환자예후불가；가작위평고HCC악성정도적생물학지표。
Objective To investigate the expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase ( PFKFB3) in hepatocellular carcinoma( HCC) and its clinical significance. Methods The expressions of PFKFB3 mRNA of tissue sections from 42 HCC and paired adjacent tissues were detected by RT-PCR. Then the relationships between PFKFB3 expression and clinicopathological variables ( gender, age, tumor size, lymphatic metastasis, cancer embolus in vein, AFP level before treatment, differentiation and me-tastasis in liver) were analyzed. Meanwhile, the difference of overall survival of patients with different expression of PFKFB3 were stud-ied by survival analysis of Kaplan-meier. Results The positive expression rate and relative level of PFKFB3 in HCC tissue were 73?81% (31/42) and 1?075±0?171, higher than 52?38% (22/42) and 0?877±0?451 in adjacent tissues with significant difference ( P<0?05) . The PFKFB3 expression is related with TNM stage, degree of differentiation, intrahepatic metastasis and overall survival ( P<0?05) . Conclusion PFKFB3 were related to carcinogenesis, development and metastasis of HCC. The expression of PFKFB3 can influence the differentiation, metastasis andprognosis of HCC, indicating it may be used as biological indicators to evaluate the degree of malignancy of HCC.
