临床肿瘤学杂志
臨床腫瘤學雜誌
림상종류학잡지
CHINESE CLINICAL ONCOLOGY
2014年
6期
490-493
,共4页
张缨%贾绍昌%项方%陆雷%王轩
張纓%賈紹昌%項方%陸雷%王軒
장영%가소창%항방%륙뢰%왕헌
肝癌%miR-21%PTEN%表达
肝癌%miR-21%PTEN%錶達
간암%miR-21%PTEN%표체
Hepatocarcinoma%miR-21%PTEN%Expression
目的：探讨miR-21在肝癌细胞中的表达水平及其与PETN的关系。方法采用荧光定量PCR分别检测人肝细胞株WRL-68和肝癌细胞株MHCC97-H、SMMC-7721中miR-21和PTEN的表达；建立人肝癌裸鼠皮下移植瘤模型，给予miR-21抑制剂后，检测移植瘤中PTEN蛋白表达和下游信号p-Akt蛋白表达。结果人肝细胞株WRL-68和肝癌细胞株MH-CC97-H、SMMC-7721中miR-21的相对表达量（以U6为内参基因）分别为2?97±0?79、137?66±23?60、11?21±1?14，PTEN相对表达量分别为2?88±0?93、0?19±0?07、0?62±0?06；3种细胞转染miR-21抑制剂后，miR-21的相对表达量分别为1?29±0?33、36?90±11?84、6?22±0?85，PTEN相对表达量分别为6?11±1?07、2?65±0?66、4?32±0?84。人肝癌裸鼠皮下移植瘤模型给予miR-21抑制剂后，MHHC97-H细胞中PTEN表达阳性，p-Akt表达下调。结论 miR-21是PTEN的上游调控因子，调节miR-21能引起PTEN的改变，继而调控Akt信号途径和细胞的增殖活性。
目的：探討miR-21在肝癌細胞中的錶達水平及其與PETN的關繫。方法採用熒光定量PCR分彆檢測人肝細胞株WRL-68和肝癌細胞株MHCC97-H、SMMC-7721中miR-21和PTEN的錶達；建立人肝癌裸鼠皮下移植瘤模型，給予miR-21抑製劑後，檢測移植瘤中PTEN蛋白錶達和下遊信號p-Akt蛋白錶達。結果人肝細胞株WRL-68和肝癌細胞株MH-CC97-H、SMMC-7721中miR-21的相對錶達量（以U6為內參基因）分彆為2?97±0?79、137?66±23?60、11?21±1?14，PTEN相對錶達量分彆為2?88±0?93、0?19±0?07、0?62±0?06；3種細胞轉染miR-21抑製劑後，miR-21的相對錶達量分彆為1?29±0?33、36?90±11?84、6?22±0?85，PTEN相對錶達量分彆為6?11±1?07、2?65±0?66、4?32±0?84。人肝癌裸鼠皮下移植瘤模型給予miR-21抑製劑後，MHHC97-H細胞中PTEN錶達暘性，p-Akt錶達下調。結論 miR-21是PTEN的上遊調控因子，調節miR-21能引起PTEN的改變，繼而調控Akt信號途徑和細胞的增殖活性。
목적：탐토miR-21재간암세포중적표체수평급기여PETN적관계。방법채용형광정량PCR분별검측인간세포주WRL-68화간암세포주MHCC97-H、SMMC-7721중miR-21화PTEN적표체；건립인간암라서피하이식류모형，급여miR-21억제제후，검측이식류중PTEN단백표체화하유신호p-Akt단백표체。결과인간세포주WRL-68화간암세포주MH-CC97-H、SMMC-7721중miR-21적상대표체량（이U6위내삼기인）분별위2?97±0?79、137?66±23?60、11?21±1?14，PTEN상대표체량분별위2?88±0?93、0?19±0?07、0?62±0?06；3충세포전염miR-21억제제후，miR-21적상대표체량분별위1?29±0?33、36?90±11?84、6?22±0?85，PTEN상대표체량분별위6?11±1?07、2?65±0?66、4?32±0?84。인간암라서피하이식류모형급여miR-21억제제후，MHHC97-H세포중PTEN표체양성，p-Akt표체하조。결론 miR-21시PTEN적상유조공인자，조절miR-21능인기PTEN적개변，계이조공Akt신호도경화세포적증식활성。
Objective To investigate and analyze the expression of miR-21 in hepatocarcinoma cell strains and the relation-ship with PTEN. Methods The quantitative reverse transcription-PCR were used to detect the expression of miR-122 and PTEN in hepatocarcinoma cell strains, PTEN and p-Akt expression was detected in tumor-bearing nude mice treated with miR-21 inhibitor. Re-sults The relative expression of miR-21 was 2?97±0?79,137?66±23?60 and 11?21±1?14 in WRL-68, MHCC97-H and SMMC-7721strains respectively, while that was 1?29 ± 0?33, 36?90 ± 11?84 and 6?22 ± 0?85 respectively when miR-21 inhibitor was transfected. The relative expression of PTEN was 2?88 ± 0?93, 0?19 ± 0?07 and 0?62 ± 0?06 in WRL-68, MHCC97-H and SMMC-7721strains respectively, while that was 6?11±1?07, 2?65±0?66 and 4?32±0?84 respectively when miR-21 inhibitor was transfected. The expression of PTEN was positive and p-Akt was down regulated when tumor-bearing nude mice was treated with miR-21 inhibitor. Conclusion MiR-21 is an upstream regulation factor of PTEN, it can affect PTEN expression.
