临床误诊误治
臨床誤診誤治
림상오진오치
CLINICAL MISDIAGNOSIS & MISTHERAPY
2014年
6期
111-115
,共5页
王宝凯%付钰%袁之林%杨超%王霜%赵吉平
王寶凱%付鈺%袁之林%楊超%王霜%趙吉平
왕보개%부옥%원지림%양초%왕상%조길평
肺%肠%哮喘%大鼠%针刺%膜蛋白质类%RNA,信使
肺%腸%哮喘%大鼠%針刺%膜蛋白質類%RNA,信使
폐%장%효천%대서%침자%막단백질류%RNA,신사
Lung%Intestine%Asthma%Rat%Acupuncture%Membrane proteins%RNA,messenger
目的探讨针刺对过敏性哮喘(哮喘)大鼠肺肠组织表面蛋白A( SP-A) mRNA表达的影响。方法将40只SD大鼠随机分为空白组、哮喘模型组、针刺肺组、针刺肠组、针刺肺肠组,每组各8只。除空白组外,余4组采用腹腔注射卵蛋白致敏,2周后尾静脉注射卵蛋白激发哮喘发作进行造模。空白组、哮喘模型组不针刺,针刺肺组针刺尺泽、孔最、列缺、肺俞穴,针刺肠组针刺曲池、合谷、天枢、上巨虚穴,针刺肺肠组针刺孔最、列缺、曲池、天枢穴。检测并比较各组大鼠针刺后肺肠等组织中SP-A mRNA的表达。结果哮喘模型组肺、大肠及小肠组织中SP-A mRNA的表达均较空白组高(P<0.01);针刺肺组、针刺肠组、针刺肺肠组的肺、大肠及针刺肺肠组小肠组织中SP-A mRNA的表达均较哮喘模型组高(P<0.05);针刺肺组、针刺肠组的小肠组织中SP-A mRNA的表达与哮喘模型组比较无明显差异(P均>0.05)。结论哮喘大鼠肺肠组织SP-A mRNA表达均发生异常,其中肺与大肠生物学相关性最为密切。
目的探討針刺對過敏性哮喘(哮喘)大鼠肺腸組織錶麵蛋白A( SP-A) mRNA錶達的影響。方法將40隻SD大鼠隨機分為空白組、哮喘模型組、針刺肺組、針刺腸組、針刺肺腸組,每組各8隻。除空白組外,餘4組採用腹腔註射卵蛋白緻敏,2週後尾靜脈註射卵蛋白激髮哮喘髮作進行造模。空白組、哮喘模型組不針刺,針刺肺組針刺呎澤、孔最、列缺、肺俞穴,針刺腸組針刺麯池、閤穀、天樞、上巨虛穴,針刺肺腸組針刺孔最、列缺、麯池、天樞穴。檢測併比較各組大鼠針刺後肺腸等組織中SP-A mRNA的錶達。結果哮喘模型組肺、大腸及小腸組織中SP-A mRNA的錶達均較空白組高(P<0.01);針刺肺組、針刺腸組、針刺肺腸組的肺、大腸及針刺肺腸組小腸組織中SP-A mRNA的錶達均較哮喘模型組高(P<0.05);針刺肺組、針刺腸組的小腸組織中SP-A mRNA的錶達與哮喘模型組比較無明顯差異(P均>0.05)。結論哮喘大鼠肺腸組織SP-A mRNA錶達均髮生異常,其中肺與大腸生物學相關性最為密切。
목적탐토침자대과민성효천(효천)대서폐장조직표면단백A( SP-A) mRNA표체적영향。방법장40지SD대서수궤분위공백조、효천모형조、침자폐조、침자장조、침자폐장조,매조각8지。제공백조외,여4조채용복강주사란단백치민,2주후미정맥주사란단백격발효천발작진행조모。공백조、효천모형조불침자,침자폐조침자척택、공최、렬결、폐유혈,침자장조침자곡지、합곡、천추、상거허혈,침자폐장조침자공최、렬결、곡지、천추혈。검측병비교각조대서침자후폐장등조직중SP-A mRNA적표체。결과효천모형조폐、대장급소장조직중SP-A mRNA적표체균교공백조고(P<0.01);침자폐조、침자장조、침자폐장조적폐、대장급침자폐장조소장조직중SP-A mRNA적표체균교효천모형조고(P<0.05);침자폐조、침자장조적소장조직중SP-A mRNA적표체여효천모형조비교무명현차이(P균>0.05)。결론효천대서폐장조직SP-A mRNA표체균발생이상,기중폐여대장생물학상관성최위밀절。
Objective To explore the influence of acupuncture on the expression of pulmonary surfactant protein (SP-A) mRNA in the lung and intestine tissues of rats with allergic asthma. Methods A total of 40 SD rats were randomly divided into control group (n=8), allergic asthma model group (n=8), the lung acupuncture group (n=8), the intestine acupuncture group (n=8) and the lung and intestine acupuncture group (n=8). Except in the control group, rats in the rest four groups re-ceived egg album by intraperitoneal injection to induce allergy, and the models of allergic asthma were established by egg albu-min of intravenous injection in vena caudalis. Rats in control and allergic asthma model groups were not treated. Rats in lung ac-upuncture group received acupuncture treatment in acu-points of Chize ( LU11 ) , Kongzui ( LU6 ) , Lieque ( LU7 ) and Feishu (BL13);rats in intestine acupuncture group received acupuncture treatment in acu-points of Quchi (LI11), Hegu (LI4), Tian-shu (ST25) and Shangjuxu (ST37); rats in the lung and intestine acupuncture group received acupuncture treatment in acu-points of Kongzui (LU6), Lieque (LU7), Quchi (LI11) and Tianshu (ST25). The SP-A mRNA expressions were detected and compared in the five groups after the treatment. Results The expressions of SP-A mRNA in the tissues of lung, large intestine and small intestine in the allergic asthma model groups were significant higher than those in control group (P<0. 01);the ex-pressions of SP-A mRNA in the tissues of lung and large intestine in the lung acupuncture, the intestine acupuncture and the lung and intestine acupuncture groups and small intestine tissues in the lung and intestine acupuncture group were significantly higher than those in the allergic asthma model group (P<0. 05);the differences in expressions of SP-A mRNA in small intes-tine tissues between the lung acupuncture and the intestine acupuncture groups with allergic asthma model group showed no sta-tistical significances (P>0. 05). Conclusion The SP-A mRNA expressions in the lung and intestine tissues of rats with aller-gic asthma are of bnormality, and the lung has significant biological relationship with the large intestine.