CAJ | 학술논문

目的：探讨不同程度肥胖儿童的生化指标和脂肪含量与肥胖相关基因（FTO）rs9936609单核苷酸多态性位点（SNP）之间的相关性。方法纳入153例7~11岁儿童，其中肥胖102例、超重51例作为研究对象；160例正常体质量儿作为正常对照组。检测并比较各组间肝功能及其他生化指标的差异；利用PCR直接测序法检测FTO基因rs9939609的SNP，计算等位基因分布频率。结果 FTO基因rs9939609 TT、TA/AA基因型在肥胖、超重和正常三组间的分布差异有统计学意义（χ2=23.01，P<0.001）；其中肥胖组和超重组TA/AA基因型表达频率均分别高于对照组，差异有统计学意义（P’<0.014）。T、A等位基因在正常对照组、超重组和肥胖组的频率分别为96.25%和3.75%，85.29%和14.71%，85.78%和14.22%，三组间的分布差异有统计学意义（χ2=21.72，P<0.001）；肥胖组和超重组A等位基因频率均分别高于对照组，差异有统计学意义（P’<0.014）。TA、AA基因型携带者体质指数（BMI）高于TT型携带者，差异有统计学意义（P<0.05）。结论 FTO基因rs9939609多态性和儿童肥胖的发生与发展有相关性，A等位基因可导致携带者BMI升高。
목적：탐토불동정도비반인동적생화지표화지방함량여비반상관기인（FTO）rs9936609단핵감산다태성위점（SNP）지간적상관성。방법납입153례7~11세인동，기중비반102례、초중51례작위연구대상；160례정상체질량인작위정상대조조。검측병비교각조간간공능급기타생화지표적차이；이용PCR직접측서법검측FTO기인rs9939609적SNP，계산등위기인분포빈솔。결과 FTO기인rs9939609 TT、TA/AA기인형재비반、초중화정상삼조간적분포차이유통계학의의（χ2=23.01，P<0.001）；기중비반조화초중조TA/AA기인형표체빈솔균분별고우대조조，차이유통계학의의（P’<0.014）。T、A등위기인재정상대조조、초중조화비반조적빈솔분별위96.25%화3.75%，85.29%화14.71%，85.78%화14.22%，삼조간적분포차이유통계학의의（χ2=21.72，P<0.001）；비반조화초중조A등위기인빈솔균분별고우대조조，차이유통계학의의（P’<0.014）。TA、AA기인형휴대자체질지수（BMI）고우TT형휴대자，차이유통계학의의（P<0.05）。결론 FTO기인rs9939609다태성화인동비반적발생여발전유상관성，A등위기인가도치휴대자BMI승고。
Objective To investigate the relationship of single nucleotide polymorphism (SNP) on FTO gene rs9939609 with metabolism index and obesity in children. Methods One hundred and fifty-three children (age 7-11 year) were recruited in this study, 102 of them were obese and 51 of them were overweight. One hundred and sixty children with normal body weight were recruited as control. Height, weight and biochemical indicator of liver function were measured. PCR and direct sequence were applied to detect the polymorphism of rs9939609, and the frequency of the allele was calculated. Results TT or TA/AA genotype frequency on FTOgene rs9939609 was significantly different among overweight group, obesity group and normal con-trol group (χ2=23.01, P<0.001);TA/AA genotype frequency in overweight and obesity group was significant higher than that in the control group(P<0.014). The frequencies of T and A allele in overweight group, obesity group and control group was 96.25%and 3.75%, 85.29%and 14.71%, 85.78%and 14.22%respectively. There was significant difference of allelic frequency among overweight group, obesity group and control group (χ2=21.72, P<0.001). The frequency of A allele in overweight and obe-sity group was higher than that in control group (P'<0.014). Subjects with TA/AA allele had significantly higher BMI compared with subjects with TT allele. Conclusions rs9939609 of FTO gene is associated with obesity in children, and allele A on this spot may raise BMI and leads to overweight and obesity.